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Dr Tina Summerfield, Senior Lecturer

summerfield_lab_photo_2015

Tina Summerfield (far right) and members of her lab group.

Cyanobacteria
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Email: tina.summerfield@otago.ac.nz
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Phone: +64 3 479 7578

Research Interests

Cyanobacterial diversity, genetics and ecophysiology, the potential of cyanobacteria as a renewable energy source and cyanobacterial symbioses.

Teaching Involvement

BIOL123 Biology of Plants
BTNY203 Marine & Freshwater Botany
BTNY465 Plants and Environment
GENE223 Developmental and Applied Genetics
GENE411 Current Topics in Genetics
PLBI301 Applied Plant Science
PLBI401 Special Topics in Plant Biotechnology

Current Research

Our research is focused on understanding the diversity and metabolic flexibility of cyanobacteria, and these microbes are found in almost all environments on Earth. They are an excellent model system for the study of photosynthesis, nitrogen assimilation, circadian rhythms, and response to environmental stresses and have potential as a renewable energy source. Less than 20% of described species have been cultured, indicating the potential of cyanobacteria remains to be discovered.

Please contact me if you are interested in research projects in any of these areas.

The Photosynthetic electron transport chain under low oxygen conditions

We have identified changes in the photosynthetic electron transport chain under low oxygen conditions that are associated with hydrogen production. We are investigating the impact of these changes on photosynthetic performance.

Cyanobacterial symbioses

Cyanobacteria form symbiotic associations with plants and fungi. We are studying cyanolichens, in particular the mode of cellular communication between the symbiotic partners and how this alters photosynthetic metabolism.

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Publications

Ludwig, L. R., Kantvilas, G., Nilsen, A. R., Orlovich, D. A., Ohmura, Y., Summerfield, T. C., … Lord, J. M. (2020). A molecular-genetic reassessment of the circumscription of the lichen genus Icmadophila. Lichenologist, 52(3), 213-220. doi: 10.1017/S0024282920000122

Nilsen, A. R., Wang, X. Y., Soop, K., Cooper, J. A., Ridley, G. S., Wallace, M., Summerfield, T. C., Brown, C. M., & Orlovich, D. A. (2020). Purple haze: Cryptic purple sequestrate Cortinarius in New Zealand. Mycologia. Advance online publication. doi: 10.1080/00275514.2020.1730120

Sheridan, K. J., Duncan, E. J., Eaton-Rye, J. J., & Summerfield, T. C. (2020). The diversity and distribution of D1 proteins in cyanobacteria. Photosynthesis Research. Advance online publication. doi: 10.1007/s11120-020-00762-7

Fagerlund, R. D., Forsman, J. A., Biswas, S., Vass, I., Davies, F. K., Summerfield, T. C., & Eaton-Rye, J. J. (2020). Stabilization of Photosystem II by the PsbT protein impacts photodamage, repair and biogenesis. Biochimica et Biophysica Acta: Bioenergetics. Advance online publication. doi: 10.1016/j.bbabio.2020.148234

Nilsen, A. R., Teasdale, S. E., Guy, P. L., Summerfield, T. C., & Orlovich, D. A. (2020). Fungal diversity in canopy soil of silver beech, Nothofagus menziesii (Nothofagaceae). PLoS ONE, 15(1), e0227860. doi: 10.1371/journal.pone.0227860

Chapter in Book - Research

Summerfield, T. C., Toepel, J., & Sherman, L. A. (2012). Transcriptional response to low-O2 conditions in Synechocystis sp. strain PCC 6803 includes induction of a gene cluster containing psbA and petC2. In S. Itoh, P. Mohanty & K. N. Guruprasad (Eds.), Photosynthesis: Overviews on recent progress and future perspectives. (pp. 120-129). New Delhi, India: I. K. International.

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Journal - Research Article

Ludwig, L. R., Kantvilas, G., Nilsen, A. R., Orlovich, D. A., Ohmura, Y., Summerfield, T. C., … Lord, J. M. (2020). A molecular-genetic reassessment of the circumscription of the lichen genus Icmadophila. Lichenologist, 52(3), 213-220. doi: 10.1017/S0024282920000122

Nilsen, A. R., Teasdale, S. E., Guy, P. L., Summerfield, T. C., & Orlovich, D. A. (2020). Fungal diversity in canopy soil of silver beech, Nothofagus menziesii (Nothofagaceae). PLoS ONE, 15(1), e0227860. doi: 10.1371/journal.pone.0227860

Fagerlund, R. D., Forsman, J. A., Biswas, S., Vass, I., Davies, F. K., Summerfield, T. C., & Eaton-Rye, J. J. (2020). Stabilization of Photosystem II by the PsbT protein impacts photodamage, repair and biogenesis. Biochimica et Biophysica Acta: Bioenergetics. Advance online publication. doi: 10.1016/j.bbabio.2020.148234

Sheridan, K. J., Duncan, E. J., Eaton-Rye, J. J., & Summerfield, T. C. (2020). The diversity and distribution of D1 proteins in cyanobacteria. Photosynthesis Research. Advance online publication. doi: 10.1007/s11120-020-00762-7

Nilsen, A. R., Wang, X. Y., Soop, K., Cooper, J. A., Ridley, G. S., Wallace, M., Summerfield, T. C., Brown, C. M., & Orlovich, D. A. (2020). Purple haze: Cryptic purple sequestrate Cortinarius in New Zealand. Mycologia. Advance online publication. doi: 10.1080/00275514.2020.1730120

Nilsen, A. R., Brown, C. M., Summerfield, T. C., & Orlovich, D. A. (2019). Cortinarius atropileatus sp. nov. (Cortinariaceae) from New Zealand. New Zealand Journal of Botany, 57(1), 50-61. doi: 10.1080/0028825X.2018.1548493

Salis, R. K., Bruder, A., Piggott, J. J., Summerfield, T. C., & Matthaei, C. D. (2019). Multiple-stressor effects of dicyandiamide (DCD) and agricultural stressors on trait-based responses of stream benthic algal communities. Science of the Total Environment, 693, 133305. doi: 10.1016/j.scitotenv.2019.07.111

Morris, J. N., Kovács, S., Vass, I., Summerfield, T. C., & Eaton-Rye, J. J. (2019). Environmental pH and Glu364 to Gln mutation in the chlorophyll-binding CP47 protein affect redox-active TyrD and charge recombination in Photosystem II. FEBS Letters, 593, 163-174. doi: 10.1002/1873-3468.13307

Ludwig, L., Summerfield, T. C., Lord, J. M., & Singh, G. (2017). Characterization of the mating-type locus (MAT) reveals a heterothallic mating system in Knightiella splachnirima. Lichenologist, 49(4), 373-385. doi: 10.1017/S0024282917000214

Morris, J. N., Eaton-Rye, J. J., & Summerfield, T. C. (2017). Phenotypic variation in wild-type substrains of the model cyanobacterium Synechocystis sp. PCC 6803. New Zealand Journal of Botany, 55(1), 25-35. doi: 10.1080/0028825X.2016.1231124

Salis, R. K., Bruder, A., Piggott, J. J., Summerfield, T. C., & Matthaei, C. D. (2017). High-throughput amplicon sequencing and stream benthic bacteria: Identifying the best taxonomic level for multiple-stressor research. Scientific Reports, 7, 44657. doi: 10.1038/srep44657

Griffith, J. C., Lee, W. G., Orlovich, D. A., & Summerfield, T. C. (2017). Contrasting bacterial communities in two indigenous Chionochloa (Poaceae) grassland soils in New Zealand. PLoS ONE, 12(6), e0179652. doi: 10.1371/journal.pone.0179652

Crawford, T. S., Hanning, K. R., Chua, J. P. S., Eaton-Rye, J. J., & Summerfield, T. C. (2016). Comparison of D1´- and D1-containing PS II reaction centre complexes under different environmental conditions in Synechocystis sp. PCC 6803. Plant, Cell & Environment, 39, 1715-1726. doi: 10.1111/pce.12738

Crawford, T. S., Eaton-Rye, J. J., & Summerfield, T. C. (2016). Mutation of Gly195 of the ChlH subunit of Mg-chelatase reduces chlorophyll and further disrupts PS II assembly in a Ycf48-deficient strain of Synechocystis sp. PCC 6803. Frontiers in Plant Science, 7, 1060. doi: 10.3389/fpls.2016.01060

Secker, N. H., Chua, J. P. S., Laurie, R. E., McNoe, L., Guy, P. L., Orlovich, D. A., & Summerfield, T. C. (2016). Characterization of the cyanobacteria and associated bacterial community from an ephemeral wetland in New Zealand. Journal of Phycology, 52(5), 761-773. doi: 10.1111/jpy.12434

Morris, J. N., Eaton-Rye, J. J., & Summerfield, T. C. (2016). Environmental pH and the requirement for the extrinsic proteins of Photosystem II in the function of cyanobacterial photosynthesis. Frontiers in Plant Science, 7, 1135. doi: 10.3389/fpls.2016.01135

Morris, J. N., Crawford, T. S., Jeffs, A., Stockwell, P. A., Eaton-Rye, J. J., & Summerfield, T. C. (2014). Whole genome re-sequencing of two ‘wild-type’ strains of the model cyanobacterium Synechocystis sp. PCC 6803. New Zealand Journal of Botany, 52(1), 36-47. doi: 10.1080/0028825X.2013.846267

Chua, J. P. S., Orlovich, D. A., & Summerfield, T. C. (2014). Cyanobacteria in New Zealand indigenous grasslands. New Zealand Journal of Botany, 52(1), 100-115. doi: 10.1080/0028825X.2013.862554

Luo, H., Jackson, S. A., Fagerlund, R. D., Summerfield, T. C., & Eaton-Rye, J. J. (2014). The importance of the hydrophilic region of PsbL for the plastoquinone electron acceptor complex of Photosystem II. Biochimica et Biophysica Acta: Bioenergetics, 1837(9), 1435-1446. doi: 10.1016/j.bbabio.2014.02.015

Wall, J. M., Wood, S. A., Orlovich, D. A., Rhodes, L. L., & Summerfield, T. C. (2014). Characterisation of freshwater and marine cyanobacteria in the Hokianga region, Northland, New Zealand. New Zealand Journal of Marine & Freshwater Research, 48(2), 177-193. doi: 10.1080/00288330.2013.854814

Summerfield, T. C., Crawford, T. S., Young, R. D., Chua, J. P. S., Macdonald, R. L., Sherman, L. A., & Eaton-Rye, J. J. (2013). Environmental pH affects photoautotrophic growth of Synechocystis sp. PCC 6803 strains carrying mutations in the lumenal proteins of PSII. Plant & Cell Physiology, 54(6), 859-874. doi: 10.1093/pcp/pct036

Chua, J. P. S., Wallace, E. J. S., Yardley, J. A., Duncan, E. J., Dearden, P. K., & Summerfield, T. C. (2012). Gene expression indicates a zone of heterocyst differentiation within the thallus of the cyanolichen Pseudocyphellaria crocata. New Phytologist, 196(3), 862-872. doi: 10.1111/j.1469-8137.2012.04272.x

Summerfield, T. C., Nagarajan, S., & Sherman, L. A. (2011). Gene expression under low-oxygen conditions in the cyanobacterium Synechocystis sp. PCC 6803 demonstrates Hik31 -dependent and -independent responses. Microbiology, 157(2), 301-312. doi: 10.1099/mic.0.041053-0

Toepel, J., McDermott, J. E., Summerfield, T. C., & Sherman, L. A. (2009). Transcriptional analysis of the unicellular, diazotrophic cyanobacterium Cyanothece sp. ATCC 51142 grown under short day/night cycles. Journal of Phycology, 45(3), 610-620. doi: 10.1111/j.1529-8817.2009.00674.x

Shcolnick, S., Summerfield, T. C., Reytman, L., Sherman, L. A., & Keren, N. (2009). The mechanism of iron homeostasis in the unicellular cyanobacterium Synechocystis sp. PCC 6803 and its relationship to oxidative stress. Plant Physiology, 150(4), 2045-2056. doi: 10.1104/pp.109.141853

Summerfield, T. C., & Sherman, L. A. (2008). Global transcriptional response of the alkali-tolerant cyanobacterium Synechocystis sp. strain PCC 6803 to a pH 10 environment. Applied & Environmental Microbiology, 74(17), 5276-5284. doi: 10.1128/AEM.00883-08

Toepel, J., Welsh, E., Summerfield, T. C., Pakrasi, H. B., & Sherman, L. A. (2008). Differential transcriptional analysis of the cyanobacterium Cyanothece sp. strain ATCC 51142 during light-dark and continuous-light growth. Journal of Bacteriology, 190(11), 3904-3913. doi: 10.1128/JB.00206-08

Summerfield, T. C., Toepel, J., & Sherman, L. A. (2008). Low-oxygen induction of normally cryptic psbA genes in cyanobacteria. Biochemistry, 47, 12939-12941.

Summerfield, T. C., & Sherman, L. A. (2007). Role of sigma factors in controlling global gene expression in light/dark transitions in the cyanobacterium Synechocystis sp. strain PCC 6803. Journal of Bacteriology, 189(21), 7829-7840. doi: 10.1128/JB.01036-07

Summerfield, T. C., Eaton-Rye, J. J., & Sherman, L. A. (2007). Global gene expression of a ΔPsbO:ΔPsbU mutant and a spontaneous revertant in the cyanobacterium Synechocystis sp. strain PCC 6803. Photosynthesis Research, 94, 265-274.

Summerfield, T. C., & Eaton-Rye, J. J. (2006). Pseudocyphellaria crocata, P. neglecta and P. perpetua from the Northern and Southern Hemispheres are a phylogenetic species and share cyanobionts. New Phytologist, 170, 597-607.

Singh, A. K., Summerfield, T. C., Li, H., & Sherman, L. A. (2006). The heat shock response in the cyanobacterium Synechocystis sp. strain PCC 6803 and regulation of gene expression by HrcA and SigB. Archives of Microbiology, 186(4), 273-286. doi: 10.1007/s00203-006-0138-0

Summerfield, T. C., Shand, J. A., Bentley, F. K., & Eaton-Rye, J. J. (2005). PsbQ (SII1638) in Synechocystis sp. PCC 6803 is required for photosystem II activity in specific mutants and in nutrient-limiting conditions. Biochemistry, 44, 805-815.

Summerfield, T. C., Winter, R. T., & Eaton-Rye, J. J. (2005). Investigation of a requirement for the PsbP-like protein in Synechocystis sp. PCC 6803. Photosynthesis Research, 84, 263-268.

Summerfield, T., Galloway, D. J., & Eaton-Rye, J. J. (2002). Species of cyanolichens from Pseudocyphellaria with indistinguishable ITS sequences have different photobionts. New Phytologist, 155, 121-129.

Mouchel, N., Tebbutt, S. J., Broackes-Carter, F. C., Sahota, V., Summerfield, T., Gregory, D. J., & Harris, A. (2001). The sheep genome contributes to localization of control elements in a human gene with complex regulatory mechanisms. Genomics, 76, 9-13.

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Journal - Research Other

Allen, R., Hoffmann, L. J., Law, C. S., & Summerfield, T. C. (2020). Subtle bacterioplankton community responses to elevated CO2 and warming in the oligotrophic South Pacific Gyre [Brief report]. Environmental Microbiology Reports. Advance online publication. doi: 10.1111/1758-2229.12844

Eaton-Rye, J. J., Guieysse, B., Packer, M. A., Summerfield, T. C., & Wood, S. A. (2019). Biology and biotechnological applications of microalgae and photosynthetic prokaryotes: Part 1. New Zealand Journal of Botany, 57(2), 65-69. doi: 10.1080/0028825X.2019.1625558

Summerfield, T. C. (2013). [Review of the book Plastid biology, energy conversion and carbon assimilation (Advances in photosynthesis and respiration, Vol. 34)]. New Zealand Journal of Botany, 51(2), 145-146. doi: 10.1080/0028825X.2012.757755

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Journal - Professional & Other Non-Research Articles

Packer, M. A., Eaton-Rye, J. J., Summerfield, T. C., Guieysse, B., & Wood, S. A. (2017). Introduction: Proceedings of the 2015 New Zealand symposium on algae and photosynthetic prokaryotes. New Zealand Journal of Botany, 55, 1-4. doi: 10.1080/0028825X.2016.1261908

Eaton-Rye, J. J., Packer, M. A., Summerfield, T. C., & Wood, S. A. (2014). Algal and cyanobacterial bioenergy and diversity. New Zealand Journal of Botany, 52(1), 1-5. doi: 10.1080/0028825X.2013.877947

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Conference Contribution - Published proceedings: Full paper

Summerfield, T. C., Young, R., Sherman, L. A., & Eaton-Rye, J. J. (2013). Characterization of a pH-sensitive Photosystem II mutant in the cyanobacterium Synechocystis sp. PCC 6803. In T. Kuang, C. Lu & L. Zhang (Eds.), Photosynthesis Research for Food, Fuel and the Future: 15th International Conference on Photosynthesis. (pp. 348-352). Berlin, Germany: Springer. doi: 10.1007/978-3-642-32034-7_73

Summerfield, T. C., & Eaton-Rye, J. J. (2005). Removal of the PsbQ-like protein (SII1638) in nutrient-limiting conditions and in strains lacking PsbO, PsbU, or PsbV. In A. van der Est & D. Bruce (Eds.), Proceedings of the 13th International Congress of Photosynthesis. 1, (pp. 336-338). Lawrence, KS, USA: Alliance Communications Group. [Full Paper]

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Conference Contribution - Published proceedings: Abstract

Sheridan, K. J., Eaton-Rye, J. J., & Summerfield, T. C. (2019). Characterisation of a phylogenetically distinct group of D1 proteins from cyanobacteria. In S. Allakhverdiev & I. Naydov (Eds.), Proceedings of the 10th International Photosynthesis and Hydrogen Energy Research for Sustainability Conference. (pp. 68). Retrieved from https://icprs.ru

Summerfield, T. C., Morris, J. N., Pearson, T., Sheridan, K., & Eaton-Rye, J. J. (2019). Characterisation of pH sensitive Photosystem II mutants in Synechocystis sp. PCC 6803. In S. Allakhverdiev & I. Naydov (Eds.), Proceedings of the 10th International Photosynthesis and Hydrogen Energy Research for Sustainability Conference. (pp. 141). Retrieved from https://icprs.ru

Orlovich, D. A., Nilsen, A. R., Kahlert, B. C., Teasdale, S. E., Guy, P. L., & Summerfield, T. C. (2018). Microbial diversity in aerial "canopy soil" microhabitats. Proceedings of the New Zealand Microbiological Society (NZMS) New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) Joint Annual Conference: Microbes & Molecules. (pp. 54). Retrieved from https://www.nzmsconference.org.nz

Allen, R., Rudminat, F., Law, C., Hoffmann, L., & Summerfield, T. (2018). Bacterioplankton resistance to acidification and warming in the oligotrophic ocean. Proceedings of the 11th New Zealand Ocean Acidification Workshop. (pp. 7). Retrieved from http://nzoac.nz

Brown, C., Chyou, T.-y., Lim, C. S., Padilla, E., Nilsen, A., Liu, S., … Summerfield, T., … Orlovich, D. (2018). The functions of non-coding RNAs and elements in fungal genomes: New roles in the regulation of gene expression. Proceedings of the New Zealand Microbiological Society (NZMS) New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) Joint Annual Conference: Microbes & Molecules. (pp. 53). Retrieved from https://www.nzmsconference.org.nz

Nilsen, A., Summerfield, T., Brown, C., & Orlovich, D. (2018). Evolution of sequestrate fungi in New Zealand, with emphasis on the genus Cortinarius. Proceedings of the New Zealand Microbiological Society (NZMS) New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) Joint Annual Conference: Microbes & Molecules. (pp. 56). Retrieved from https://www.nzmsconference.org.nz

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