Dr Louisa Ashby's research is focused on the biochemistry of the neutrophil, and in particular the enzymology of myeloperoxidase. This enzyme kills microbes by generating chlorine bleach, but during inflammation it can also damage healthy tissue.
She is also interested in the oxidation chemistry of myeloperoxidase in its microbicidal activity, and how to effectively inhibit the enzyme from causing oxidative stress in disease.
Paumann-Page, M., Ashby, L. V., Khalilova, I., Magon, N. J., Hofbauer, S., Paton, L. N., … Kettle, A. J. (2023). Hypochlorous acid inactivates myeloperoxidase inside phagocytosing neutrophils. Redox Biochemistry & Chemistry, 5-6, 100008. doi: 10.1016/j.rbc.2023.100008
Ashby, L. V., Flannery, M., Chua, I., & Kettle, A. J. (2023). Measuring oxidant production in phagosomes to identify abnormalities in neutrophil function. Free Radical Biology & Medicine, 201(Suppl. 1), (pp. 13). doi: 10.1016/j.freeradbiomed.2023.03.072
Kettle, A. J., Ashby, L. V., Winterbourn, C. C., & Dickerhof, N. (2023). Superoxide: The enigmatic chemical chameleon in neutrophil biology [Invited review]. Immunological Reviews. Advance online publication. doi: 10.1111/imr.13183
Ashby, L. V., Dickerhof, N., Springer, R., Hampton, M. B., & Kettle, A. J. (2022, August). Bacterial resistance to oxidants in neutrophil phagosomes. Poster session presented at the Webster Centre for Infectious Diseases Satellite Meeting: Queenstown Research Week, Queenstown, New Zealand.
Ashby, L. V., Springer, R., Loi, V. V., Antelmann, H., Hampton, M. B., Kettle, A. J., & Dickerhof, N. (2022). Oxidation of bacillithiol during killing of Staphylococcus aureus USA300 inside neutrophil phagosomes. Journal of Leukocyte Biology, 112, 591-605. doi: 10.1002/jlb.4hi1021-538rr