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Nina DickerhofSenior Research Fellow

MSc(Germany) PhD(Otago)

Email nina.dickerhof@otago.ac.nz
Tel +64 3 364 0568

Research interests

Dr Nina Dickerhof's research is focused on neutrophil-derived oxidants and their role in health and disease. Her specialist expertise is mass spectrometry and its application to measuring oxidative modification of proteins, peptides and small molecules. She is interested in the development of biomarkers of neutrophil-derived oxidative stress in inflammatory and infectious disease with a particular focus on cystic fibrosis lung disease.

Dr Dickerhof is also studying the functional implications of oxidative modifications of proteins present at inflammatory sites, such as the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF) and the neutrophil-resident antibacterial protein calprotectin.

More recently, she has become interested in understanding how bacteria protect themselves from oxidants produced by the immune system with a special interest in the role of bacterial low molecular weight thiols.

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Publications

Shearer, H. L., Hampton, M. B., & Dickerhof, N. (2022). Bactericidal activity of the oxidants derived from mammilian heme peroxidases. In C. L. Hawkins & W. M. Nauseef (Eds.), Mammalian heme peroxidases: Diverse roles in health and disease. (pp. 171-188). Boca Raton, FL: CRC Press. doi: 10.1201/9781003212287

Schindler, L., Smyth, L. C. D., Bernhagen, J., Hampton, M. B., & Dickerhof, N. (2021). Macrophage migration inhibitory factor (MIF) enhances hypochlorous acid production in phagocytic neutrophils. Redox Biology, 41, 101946. doi: 10.1016/j.redox.2021.101946

Parker, H. A., Dickerhof, N., Forrester, L., Ryburn, H., Smyth, L., Messens, J., Aung, H. L., Cook, G. M., Kettle, A. J., & Hampton, M. B. (2021). Mycobacterium smegmatis resists the bactericidal activity of hypochlorous acid produced in neutrophil phagosomes. Journal of Immunology, 206, 1901-1912. doi: 10.4049/jimmunol.2001084

Schindler, L., Zwissler, L., Krammer, C., Hendgen-Cotta, U., Rassaf, T., Hampton, M. B., Dickerhof, N., & Bernhagen, J. (2021). Macrophage migration inhibitory factor inhibits neutrophil apoptosis by inducing cytokine release from mononuclear cells. Journal of Leukocyte Biology. Advance online publication. doi: 10.1002/JLB.3A0420-242RRR

Dickerhof, N., Paton, L., & Kettle, A. J. (2020). Oxidation of bacillithiol by myeloperoxidase-derived oxidants. Free Radical Biology & Medicine, 158, 74-83. doi: 10.1016/j.freeradbiomed.2020.06.009

Shearer, H. L., Hampton, M. B., & Dickerhof, N. (2022). Bactericidal activity of the oxidants derived from mammilian heme peroxidases. In C. L. Hawkins & W. M. Nauseef (Eds.), Mammalian heme peroxidases: Diverse roles in health and disease. (pp. 171-188). Boca Raton, FL: CRC Press. doi: 10.1201/9781003212287

Chapter in Book - Research

Parker, H. A., Dickerhof, N., Forrester, L., Ryburn, H., Smyth, L., Messens, J., Aung, H. L., Cook, G. M., Kettle, A. J., & Hampton, M. B. (2021). Mycobacterium smegmatis resists the bactericidal activity of hypochlorous acid produced in neutrophil phagosomes. Journal of Immunology, 206, 1901-1912. doi: 10.4049/jimmunol.2001084

Journal - Research Article

Schindler, L., Smyth, L. C. D., Bernhagen, J., Hampton, M. B., & Dickerhof, N. (2021). Macrophage migration inhibitory factor (MIF) enhances hypochlorous acid production in phagocytic neutrophils. Redox Biology, 41, 101946. doi: 10.1016/j.redox.2021.101946

Journal - Research Article

Schindler, L., Zwissler, L., Krammer, C., Hendgen-Cotta, U., Rassaf, T., Hampton, M. B., Dickerhof, N., & Bernhagen, J. (2021). Macrophage migration inhibitory factor inhibits neutrophil apoptosis by inducing cytokine release from mononuclear cells. Journal of Leukocyte Biology. Advance online publication. doi: 10.1002/JLB.3A0420-242RRR

Journal - Research Article

Chami, B., San Gabriel, P. T., Kum-Jew, S., Wang, X., Dickerhof, N., Dennis, J. M., & Witting, P. K. (2020). The nitroxide 4-methoxy-tempo inhibits the pathogenesis of dextran sodium sulfate-stimulated experimental colitis. Redox Biology, 28, 101333. doi: 10.1016/j.redox.2019.101333

Journal - Research Article

Dickerhof, N., Huang, J., Min, E., Michaëlsson, E., Lindstedt, E.-L., Pearson, J. F., Kettle, A. J., & Day, B. J. (2020). Myeloperoxidase inhibition decreases morbidity and oxidative stress in mice with cystic fibrosis-like lung inflammation. Free Radical Biology & Medicine, 152, 91-99. doi: 10.1016/j.freeradbiomed.2020.03.001

Journal - Research Article

Dickerhof, N., Paton, L., & Kettle, A. J. (2020). Oxidation of bacillithiol by myeloperoxidase-derived oxidants. Free Radical Biology & Medicine, 158, 74-83. doi: 10.1016/j.freeradbiomed.2020.06.009

Journal - Research Article

Kader, T., Porteous, C. M., Jones, G. T., Dickerhof, N., Narayana, V. K., Tull, D., Taraknath, S., & McCormick, S. P. A. (2020). Ribose-cysteine protects against the development of atherosclerosis in apoE-deficient mice. PLoS ONE, 15(2), e0228415. doi: 10.1371/journal.pone.0228415

Journal - Research Article

Dickerhof, N., Isles, V., and also Pattemore, P., Hampton, M. B., & Kettle, A. J. (2019). Exposure of Pseudomonas aeruginosa to bactericidal hypochlorous acid during neutrophil phagocytosis is compromised in cystic fibrosis. Journal of Biological Chemistry, 294(36), 13502-13514. doi: 10.1074/jbc.RA119.009934

Journal - Research Article

Hoskin, T. S., Crowther, J. M., Cheung, J., Epton, M. J., Sly, P. D., Elder, P. A., … Kettle, A. J., & Dickerhof, N. (2019). Oxidative cross-linking of calprotectin occurs in vivo, altering its structure and susceptibility to proteolysis. Redox Biology, 24, 101202. doi: 10.1016/j.redox.2019.101202

Journal - Research Article

Albrett, A. M., Ashby, L. V., Dickerhof, N., Kettle, A. J., & Winterbourn, C. C. (2018). Heterogeneity of hypochlorous acid production in individual neutrophil phagosomes revealed by a rhodamine-based probe. Journal of Biological Chemistry, 293(40), 15715-15724. doi: 10.1074/jbc.RA118.004789

Journal - Research Article

Khalilova, I. S., Dickerhof, N., Mocatta, T. J., Bhagra, C. J., McClean, D. R., Obinger, C., & Kettle, A. J. (2018). A myeloperoxidase precursor, pro-myeloperoxidase, is present in human plasma and elevated in cardiovascular disease patients. PLoS ONE, 13(3), e0192952. doi: 10.1371/journal.pone.0192952

Journal - Research Article

Turner, R., Brennan, S. O., Ashby, L. V., Dickerhof, N., Hamzah, M. R., Pearson, J. F., Stamp, L. K., & Kettle, A. J. (2018). Conjugation of urate-derived electrophiles to proteins during normal metabolism and inflammation. Journal of Biological Chemistry, 293(51), 19886-19898. doi: 10.1074/jbc.RA118.005237

Journal - Research Article

Dickerhof, N., Pearson, J. F., Hoskin, T. S., Berry, L. J., Turner, R., Sly, P. D., Kettle, A. J., on behalf of AREST CF. (2017). Oxidative stress in early cystic fibrosis lung disease is exacerbated by airway glutathione deficiency. Free Radical Biology & Medicine, 113, 236-243. doi: 10.1016/j.freeradbiomed.2017.09.028

Journal - Research Article

Dickerhof, N., Turner, R., Khalilova, I., Fantino, E., Sly, P. D., Kettle, A. J., on behalf of AREST CF. (2017). Oxidized glutathione and uric acid as biomarkers of early cystic fibrosis lung disease. Journal of Cystic Fibrosis, 16(2), 214-221. doi: 10.1016/j.jcf.2016.10.012

Journal - Research Article

Schindler, L., Dickerhof, N., Hampton, M. B., & Bernhagen, J. (2017). Post-translational regulation of macrophage migration inhibitory factor: Basis for functional fine-tuning. Redox Biology, 15, 135-142. doi: 10.1016/j.redox.2017.11.028

Journal - Research Article

Chandler, J. D., Min, E., Huang, J., McElroy, C. S., Dickerhof, N., Mocatta, T., … Kettle, A. J., … Day, B. J. (2015). Antiinflammatory and antimicrobial effects of thiocyanate in a cystic fibrosis mouse model. American Journal of Respiratory Cell & Molecular Biology, 53(2), 193-205. doi: 10.1165/rcmb.2014-0208OC

Journal - Research Article

Dickerhof, N., Schindler, L., Bernhagen, J., Kettle, A. J., & Hampton, M. B. (2015). Macrophage migration inhibitory factor (MIF) Is rendered enzymatically inactive by myeloperoxidase-derived oxidants but retains its immunomodulatory function. Free Radical Biology & Medicine, 89, 498-511. doi: 10.1016/j.freeradbiomed.2015.09.009

Journal - Research Article

Odobasic, D., Muljadi, R. C. M., O'Sullivan, K. M., Kettle, A. J., Dickerhof, N., Summers, S. A., … Holdsworth, S. R. (2015). Suppression of autoimmunity and renal disease in pristane-induced lupus by myeloperoxidase. Arthritis & Rheumatology, 67(7), 1868-1880. doi: 10.1002/art.39109

Journal - Research Article

Dickerhof, N., Magon, N. J., Tyndall, J. D. A., Kettle, A. J., & Hampton, M. B. (2014). Potent inhibition of macrophage migration inhibitory factor (MIF) by myeloperoxidase-dependant oxidation of epicatechins. Biochemical Journal, 462(2), 303-314. doi: 10.1042/BJ20140612

Journal - Research Article

Kettle, A. J., Albrett, A. M., Chapman, A. L., Dickerhof, N., Forbes, L. V., Khalilova, I., & Turner, R. (2014). Measuring chlorine bleach in biology and medicine. Biochimica et Biophysica Acta: General Subjects, 1840(2), 781-793. doi: 10.1016/j.bbagen.2013.07.004

Journal - Research Article

Seidel, A., Parker, H., Turner, R., Dickerhof, N., Khalilova, I. S., Wilbanks, S. M., Kettle, A. J., & Jameson, G. N. L. (2014). Uric acid and thiocyanate as competing substrates of lactoperoxidase. Journal of Biological Chemistry, 289(32), 21937-21949. doi: 10.1074/jbc.M113.544957

Journal - Research Article

Soethoudt, M., Peskin, A. V., Dickerhof, N., Paton, L. N., Pace, P. E., & Winterbourn, C. C. (2014). Interaction of adenanthin with glutathione and thiol enzymes: Selectivity for thioredoxin reductase and inhibition of peroxiredoxin recycling. Free Radical Biology & Medicine, 77, 331-339. doi: 10.1016/j.freeradbiomed.2014.09.025

Journal - Research Article

Peskin, A. V., Dickerhof, N., Poynton, R. A., Paton, L. N., Pace, P. E., Hampton, M. B., & Winterbourn, C. C. (2013). Hyperoxidation of peroxiredoxins 2 and 3: Rate constants for the reactions of the sulfenic acid of the peroxidatic cysteine. Journal of Biological Chemistry, 288, 14170-14177. doi: 10.1074/jbc.M113.460881

Journal - Research Article

Kato, Y., Peskin, A. V., Dickerhof, N., Harwood, D. T., & Kettle, A. J. (2012). Myeloperoxidase catalyzes the conjugation of serotonin to thiols via free radicals and tryptamine-4,5-dione. Chemical Research in Toxicology, 25(11), 2322-2332. doi: 10.1021/tx300218f

Journal - Research Article

Dickerhof, N., Kleffmann, T., Jack, R., & McCormick, S. (2011). Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain. FEBS Journal, 278(12), 2034-2043. doi: 10.1111/j.1742-4658.2011.08119.x

Journal - Research Article

Albrett, A. M., Ashby, L. V., Dickerhof, N., Hampton, M. B., Winterbourn, C. C., & Kettle, A. J. (2018). Detection of hypochlorous acid in neutrophil phagosomes with the probe R19S: Chlorinated product formation and real-time fluorescence. Proceedings of the New Zealand Microbiological Society (NZMS) New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) Joint Annual Conference: Microbes & Molecules. (pp. 112-113). Retrieved from https://www.nzmsconference.org.nz

Conference Contribution - Published proceedings: Abstract

Chami, B., Vallejo, A., KumJew, S., Dickerhof, N., Kettle, A. J., Dennis, J. M., & Witting, P. (2018). Role for neutrophil-derived myeloperoxidase in promoting acute colitis; inhibition of disease progression with 4-Methoxy TEMPO. Free Radical Biology & Medicine, 120(Suppl. 1), (pp. S38). doi: 10.1016/j.freeradbiomed.2018.04.131

Conference Contribution - Published proceedings: Abstract

Dickerhof, N., Berry, L. J., Turner, R., Hoskin, T., Sly, P. D., & Kettle, A. J. (2016). Glutathione deficiency and neutrophil-mediated oxidative stress in the lungs of young children with cystic fibrosis. Free Radical Biology & Medicine, 100(Suppl.), (pp. S190). doi: 10.1016/j.freeradbiomed.2016.10.519

Conference Contribution - Published proceedings: Abstract

Spencer, E., Dickerhof, N., Tyndall, J., Bernhagen, J., Kettle, A., & Hampton, M. (2015). Assessing inhibition of macrophage migration inhibitory factor by isothiocyanates. Free Radical Biology & Medicine, 86(Suppl. 1), (pp. S42-S43). doi: 10.1016/j.freeradbiomed.2015.07.145

Conference Contribution - Published proceedings: Abstract

Dickerhof, N., Kettle, A. J., & Hampton, M. B. (2013). Covalent modification of the pro-inflammatory cytokine MIF by dietary and endogenous electrophiles. Free Radical Biology & Medicine, 65(Suppl. 2), (pp. S35-S36). doi: 10.1016/j.freeradbiomed.2013.10.470

Conference Contribution - Published proceedings: Abstract

Jameson, G. N. L., Seidel, A., Parker, H., Turner, R., Dickerhof, N., Wilbanks, S. M., Kettle, A. J., Young, V. L., & Ward, V. K. (2013). Uric acid modulates the activity of lactoperoxidase. Proceedings of the New Zealand Institute of Chemistry (NZIC) Conference. Christchurch, New Zealand: NZIC. [Abstract]

Conference Contribution - Published proceedings: Abstract

Magon, N. J., Turner, R., Dickerhof, N., Gearry, R., Hampton, M., Sly, P., & Kettle, A. (2013). Oxidation of methionine in calprotectin by neutrophils in the airways of children with cystic fibrosis. Free Radical Biology & Medicine, 65(Suppl. 2), (pp. S62-S63). doi: 10.1016/j.freeradbiomed.2013.10.535

Conference Contribution - Published proceedings: Abstract

Khalilova, I. S., Dickerhof, N., Mocatta, T., & Kettle, A. J. (2012). Detection of pro-myeloperoxidase in circulation. Proceedings of the Oxygen Theme Meeting. (pp. 29). Dunedin, New Zealand: University of Otago. Retrieved from https://www.otago.ac.nz/research/otago051864.pdf

Conference Contribution - Published proceedings: Abstract

Peskin, A. V., Dickerhof, N., Poynton, R. A., Pace, P., Hampton, M. B., & Winterbourn, C. C. (2012). Kinetics of hyperoxidation of human peroxiredoxin 2. Free Radical Biology & Medicine, 53(Suppl. 1), (pp. S210-S211). doi: 10.1016/j.freeradbiomed.2012.08.441

Conference Contribution - Published proceedings: Abstract

Poynton, R., Peskin, A., Dickerhof, N., Paton, L., Winterbourn, C., & Hampton, M. (2012). Kinetics of peroxiredoxin 2 and 3 hyperoxidation. Proceedings of the Oxygen Theme Meeting. (pp. 14). Dunedin, New Zealand: University of Otago. Retrieved from https://www.otago.ac.nz/research/otago051864.pdf

Conference Contribution - Published proceedings: Abstract

Dickerhof, N., von Zychlinski-Kleffmann, A., Kleffmann, T., Jack, R., & McCormick, S. (2009). Using an old antibiotic to lower lipoprotein(a). Proceedings of the ComBio2009 Conference. [CD-ROM] [Abstract]

Conference Contribution - Published proceedings: Abstract

Dickerhof, N., Kettle, A. J., & Hampton, M. B. (2013, August). Covalent modification of the pro-inflammatory cytokine MIF by dietary and endogenous electrophiles. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Conference Contribution - Poster Presentation (not in published proceedings)

Khalilova, I. S., Dickerhof, N., Mocatta, T., & Kettle, A. J. (2013, August). Pro-myeloperoxidase is present in circulation and a potential participant in cardiovascular disease. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Conference Contribution - Poster Presentation (not in published proceedings)

Peskin, A. V., Dickerhof, N., Paton, L. N., Poynton, R. A., Pace, P., Hampton, M. B., & Winterbourn, C. C. (2013, August). Hyperoxidation of human peroxiredoxin 2 and 3. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Conference Contribution - Poster Presentation (not in published proceedings)

Poynton, R. A., Peskin, A. V., Dickerhof, N., Lowther, T. W., Betz, A., Winterbourn, C. C., & Hampton, M. B. (2013, August). Kinetics and biochemical properties of peroxiredoxin 3 hyperoxidation. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Conference Contribution - Poster Presentation (not in published proceedings)

Soethoudt, M., Peskin, A. V., Dickerhof, N., & Winterbourn, C. C. (2013, August). The diterpenoid Adenanthin favors binding to glutathione over peroxiredoxin 2 in erythrocytes. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Conference Contribution - Poster Presentation (not in published proceedings)

Spencer, E. S., Dale, E., Smith, R. A. J., Gamble, A. B., Tyndall, J. D. A., Rutledge, M. T., Wilbanks, S. M., Dickerhof, N., Kettle, A. J., & Hampton, M. B. (2013, August). Assessing inhibition of macrophage migration inhibitory factor by novel isothiocyanates. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Conference Contribution - Poster Presentation (not in published proceedings)

Dickerhof, N., Kleffmann, T., Jack, R., & McCormick, S. (2011, September). Bacitracin inhibits the reductive activity of protein disulfide isomerase by forming a disulfide bond with free cysteines in the substrate binding domain. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Conference Contribution - Poster Presentation (not in published proceedings)

Dickerhof, N., Pearson, J. F., Hoskin, T. S., Sly, P. D., & Kettle, A. J. (2017, August). Myeloperoxidase is associated with bronchiectasis in children with cystic fibrosis. Verbal presentation at the 10th International Human Peroxidase Meeting, Breckenridge, USA.

Conference Contribution - Verbal presentation and other Conference outputs

Hampton, M. B., Dickerhof, N., Pace, P. E., & Bozonet, S. M. (2017, July). Redox signalling and the regulation of immune cell life and death. Verbal presentation at the New Zealand Branch of the Australasian Society for Immunology (NZ ASI) Annual Scientific Meeting, Christchurch, New Zealand.

Conference Contribution - Verbal presentation and other Conference outputs

Hoskin, T. S., Dickerhof, N., Hampton, M. B., & Kettle, A. J. (2017, July). Calprotectin is sensitive to oxidants generated by myeloperoxidase. Verbal presentation at the New Zealand Branch of the Australasian Society for Immunology (NZ ASI) Annual Scientific Meeting, Christchurch, New Zealand.

Conference Contribution - Verbal presentation and other Conference outputs

Dickerhof, N., Fantino, E., Turner, R., Sly, P., & Kettle, A. J. (2015, December). Evaluation of glutathione sulphonamide and allantoin as blood and urine based indicators of exacerbations in children with cystic fibrosis. Verbal presentation at the Society for Free Radical Research Australasia & Japan Seventh Joint Meeting, Christchurch, New Zealand.

Conference Contribution - Verbal presentation and other Conference outputs

Dickerhof, N., Kettle, A. J., & Hampton, M. B. (2015, September). Myeloperoxidase-derived oxidants render macrophage migration inhibitory factor (MIF) enzymatically inactive while retaining its immunologic function. Verbal presentation at the 9th International Human Peroxidase Meeting, Cologne, USA.

Conference Contribution - Verbal presentation and other Conference outputs

Dickerhof, N. (2014, July). Potent inhibition of macrophage migration inhibitory factor by myeloperoxidase-derived electrophiles and oxidants. Verbal presentation at the Oxygen Theme Meeting, Christchurch, New Zealand.

Conference Contribution - Verbal presentation and other Conference outputs

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