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Contact Details

Phone
+64 3 479 9190
Email
jisha.antony@otago.ac.nz
Position
Senior Research Fellow
Department
Department of Pathology (Dunedin)
Research summary
Chromatin organisation in cancer

Research

My research focuses on understanding how leukaemia associated mutations in chromatin organisers such as cohesin, impacts chromatin folding or causes translocations such as RUNX1-RUNX1T1. Our work has identified enhancer dysfunction as the major effect of cohesin mutation.

We have also shown that the impact of aberrant enhancer activity is most profound and causes gene expression dysregulation when cells have to respond to differentiation or external signalling cues.

We are currently working on determining whether enhancer dysfunction can be combated using enhancer blocking drugs or by individual targeting of enhancers using CRISPR-Cas9 technology. I am also using genome-wide CRISPR-Cas9 screens in cohesin mutant leukaemia cells to identify synthetic lethal targets that could be therapeutically targeted.

To study the impact of cohesin mutation on signalling events we are using induced pluripotent stem cells and differentiating them to haematopoietic cells to examine chromatin organisation and gene expression. The work is funded by Cancer Society and Health Research Council grants.

Publications

Banerjee, R., Ajithkumar, P., Keestra, N., Smith, J., Gimenez, G., Rodger, E. J., Eccles, M. R., Antony, J., Weeks, R. J., & Chatterjee, A. (2024). Targeted DNA methylation editing using an all-in-one system establishes paradoxical activation of EBF3. Cancers, 16, 898. doi: 10.3390/cancers16050898 Journal - Research Article

Lynch, D. M., Meier, M., Labudina, A., Antony, J., & Horsfield, J. A. (2023). How STAG proteins influence cohesins role in cell fate determination. Proceedings of the Genetics Otago (GO) Annual Symposium & Workshops. Retrieved from https://blogs.otago.ac.nz/go Conference Contribution - Published proceedings: Abstract

Labudina, A. A., Gimenez, G., Meier, M., Ketharnathan, S., Antony, J., & Horsfield, J. A. (2023). Using a zebrafish stem cell model to understand the molecular basis of cohesinopathies. Proceedings of the Genetics Otago (GO) Annual Symposium & Workshops. Retrieved from https://blogs.otago.ac.nz/go Conference Contribution - Published proceedings: Abstract

Labudina, A. A., Gimenez, G., Meier, M., Ketharnathan, S., Antony, J., & Horsfield, J. A. (2023). Using a zebrafish stem cell model to understand the molecular basis of cohesinopathies. In T. Milne, M. Anwar, L. Burga, H. Harcombe, M. Garelja, A. Middleton, D. C. Ribeiro, N. Fleming, K. Ogbuehi & A. Bahn (Eds.), Proceedings of the 267th Otago Medical School Research Society (OMSRS) Meeting: PhD Student Speaker Awards. Dunedin, New Zealand: OMSRS. Retrieved from https://ourarchive.otago.ac.nz/handle/10523/12839 Conference Contribution - Published proceedings: Abstract

Wilson, L., Harcombe, H., Jayakaran, P., Burga, L., Antony, J., Morgaine, K., Garelja, M., Middleton, A., Anwar, M., & Milne, T. (Eds.). (2022). Proceedings of the 264th Otago Medical School Research Society (OMSRS) Meeting: Masters/Honours Student Speaker Awards. Dunedin, New Zealand: OMSRS. Retrieved from https://ourarchive.otago.ac.nz/handle/10523/12839 Conference Contribution - Edited volume of conference proceedings

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