Brillouin microscopy for cellular biomechanics

Overview

Brillouin microscopy is an emerging optical imaging technology that uses interactions between light and sound waves to map the mechanical properties of materials in three dimensions, without physical contact. This enables biomechanical measurements in otherwise inaccessible locations, including within living cells and deep inside biological tissues such as the eye lens. Despite its unique capabilities, Brillouin scattering is inherently weak, resulting in long acquisition times and complex instrumentation requirements.

This PhD project will develop a novel stimulated Brillouin microscopy approach based on advanced beam shaping to overcome these limitations. The successful candidate will contribute to both the development of the technology and its application to important questions in cellular biomechanics. Candidate’s role The successful candidate will take a hands-on role in both developing the technology and applying it to biomechanical studies.

The project may involve:

• Designing, building, and testing optical instrumentation
• Developing hardware-control and automation software
• Implementing signal-processing and data-analysis methods for extracting biomechanical parameters
• Characterising system performance using controlled experimental models
• Conducting preliminary studies on bioprinted tissues in collaboration with tissue engineers

This is a highly interdisciplinary project that combines optical physics, instrumentation, signal processing, and biomechanics. The student will work closely with researchers from multiple disciplines and institutions.

The project is led by Dr Michael Taylor (Biophotonics, University of Otago) and includes collaborations with Professor Tim Woodfield (Tissue Engineering, University of Otago Christchurch) and Professor Warwick Bowen (Optical Physics, University of Queensland). The student will also benefit from collaborations and networking opportunities through the Dodd-Walls Centre for Photonic and Quantum Technologies and the Australian Research Council Centre of Excellence in Quantum Biotechnology (QUBIC).

Selection criteria

Applicants should have a strong academic background in Physics, Engineering, Mathematics, or a related discipline. Experience in one or more of the following areas is desirable:

• Optical instrumentation or laser-based experiments
• Microscopy or biophotonics
• Scientific programming and automation
• Signal processing or data analysis

Strong written and verbal communication skills and an enthusiasm for interdisciplinary research are highly valued.

Eligibility

Both domestic and international students are welcome to apply.

Candidates must have one of the following

• An MSc degree including a research component equivalent to at least one-quarter of a full academic year
• Bachelor's degree with Honours 1st Class, or 2nd Class Division 1
• Evidence of appropriate research experience that demonstrates potential to pursue graduate research studies.

If English is not your first language, you need to demonstrate proficiency in English Host institution

The project will be based in the Department of Physics at the University of Otago in Dunedin, New Zealand. Otago is New Zealand's oldest university and is internationally recognised for excellence in research and teaching, particularly in the health sciences and physical sciences. The student will join a newly established biophotonics research group and play an important role in developing its future research directions, while benefiting from access to advanced optical instrumentation and strong national and international collaborations.

Applying

Please contact Dr Michael Taylor with expressions of interest or any questions.

Email m.taylor@otago.ac.nz

Applications should include:

• Curriculum vitae (including contact details for 2–3 referees)
• Academic transcripts
• Cover letter outlining:
  • Why you wish to undertake a PhD
  • How your previous experience has prepared you for this project
  • Your research interests and career goals

Funding

The position includes a tax-free stipend of NZD 35,000 per annum for three years, plus full tuition fee coverage.

Useful information

• Graduate research at the University of Otago
• Scholarships at the University of Otago