I completed both my Masters and PhD in Biomedical Science through Victoria University of Wellington, before undertaking a post-doctoral fellowship here at the University of Otago, Wellington campus. The main focus of my research has always been centred in developing better scoring tools for stratifying risk in cardiac disease. Identifying patients who are at increased risk of adverse clinical events means patient management can be personalised in order to improve patient outcomes.
My current research uses a mixture of molecular and genetic techniques in order to identify biomarkers relating to inflammation, small RNA molecules and genetic variants measured in patients who have suffered a heart attack. My research is done through Wellington Cardiovascular Research Group, and we work closely with cardiologists from Wellington Hospital in how we can best apply biomedical information so patients can benefit from better risk assessment models.
There are several postgraduate research opportunities (Honours, Masters, PhD) available in this field through the Wellington Cardiovascular Research Group.
Contact details
Department of Surgery & Anaesthesia University of Otago, Wellington PO Box 7343 Wellington South
Scientist, Wellington Cardiovascular Research Group
Postgraduate Biomedical Seminar Series Co-ordinator
Research expertise
Translational Cardiovascular Medicine
Publications
Yiu, J. Y. T., Hally, K. E., Larsen, P. D., & Holley, A. S. (2023). Neutrophil-enriched biomarkers and long-term prognosis in acute coronary syndrome: A systematic review and meta-analysis. Journal of Cardiovascular Translational Research. Advance online publication. doi: 10.1007/s12265-023-10425-2
Journal - Research Article
Holley, A., Yiu, J., Hally, K., & Larsen, P. (2023). Neutrophil biomarkers and long-term prognosis in acute coronary syndrome: A systematic review and meta-analysis. Heart, Lung & Circulation, 32(Suppl. 3), 678. doi: 10.1016/j.hlc.2023.06.582
Conference Contribution - Published proceedings: Abstract
Blackshaw, C., Hally, K., Harding, S., Fairley, S., Larsen, P., & Holley, A. (2023). Gender differences in demographics, management, and clinical outcomes in AMI patients. Heart, Lung & Circulation, 32(Suppl. 3), 386. doi: 10.1016/j.hlc.2023.06.382
Conference Contribution - Published proceedings: Abstract
Yiu, J. Y. T., Hally, K. E., Larsen, P. D., & Holley, A. S. (2023). Increased levels of low density neutrophils (LDNs) in myocardial infarction. Acta Cardiologica, 78(1), 47-54. doi: 10.1080/00015385.2021.2015145
Journal - Research Article
Whitfield, P., Sword, D., Holley, A., Walsh, E., Weatherall, M., Shepherd, P. R., … Hall, R. M., & Krebs, J. D. (2022). The metabolic effects of a CREBRF gene variant in NZ women. Proceedings of the 45th New Zealand Society for the Study of Diabetes (NZSSD) Annual Scientific Meeting. (pp. 46). Retrieved from https://www.ivvy.com.au/event/NZSSD22/welcome.html
Conference Contribution - Published proceedings: Abstract
Brunton-O'Sullivan, M. M., Holley, A. S., Bird, G. K., Kristono, G. A., Harding, S. A., & Larsen, P. D. (2022). Examining variation and temporal dynamics of extracellular matrix biomarkers following acute myocardial infarction. Biomarkers in Medicine, 16(3), 147-161. doi: 10.2217/bmm-2021-0531
Journal - Research Article
Brunton-O'Sullivan, M. M., Holley, A. S., Shi, B., Harding, S. A., & Larsen, P. D. (2022). Cluster analysis of extracellular matrix biomarkers predicts the development of impaired systolic function within 1 year of acute myocardial infarction. Heart & Vessels, 37, 2029-2038. doi: 10.1007/s00380-022-02118-8
Journal - Research Article
Rogan, A., Brunton-O'Sullivan, M., Holley, A., McQuade, D. d., & Larsen, P. (2022). Can serum biomarkers be used to rule out significant intracranial pathology in emergency department patients with mild traumatic brain injury? A systemic review & meta-analysis. Injury, 53, 259-271. doi: 10.1016/j.injury.2021.10.015
Journal - Research Article
Whitfield, P., Sword, D., Holley, A., Walsh, E., Weatherall, M., Shepherd, P., … Hall, R. M., & Krebs, J. D. (2022). The metabolic effects of a CREBRF gene variant in NZ women: Assessment of satiety and incretins. Proceedings of the 45th New Zealand Society for the Study of Diabetes (NZSSD) Annual Scientific Meeting. (pp. 22). Retrieved from https://www.ivvy.com.au/event/NZSSD22/welcome.html
Conference Contribution - Published proceedings: Abstract
Brunton-O'Sullivan, M. M., Holley, A. S., Hally, K. E., Kristono, G. A., Harding, S. A., & Larsen, P. D. (2021). A combined biomarker approach for characterising extracellular matrix profiles in acute myocardial infarction. Scientific Reports, 11, 12705. doi: 10.1038/s41598-021-92108-z
Journal - Research Article
Gunawardene, A., Manning, S., Holley, A., Kristono, G., Danielson, K., Shekouh, A., Larsen, P., & Dennett, E. (2021). An IL8-CEA combined score is stage-specific in colorectal cancer. Surgery, Gastroenterology & Oncology, 26(4), 284-290. doi: 10.21614/sgo-26-4-401
Journal - Research Article
Kristono, G., Holley, A., Hally, K., Brunton-O'Sullivan, M., Shi, B., Harding, S., & Larsen, P. (2021). Using principal component analysis to create prognostic cytokine scores for adverse outcome in acute myocardial infarction. Heart, Lung & Circulation, 30(Suppl. 2), (pp. S57-S58). doi: 10.1016/j.hlc.2021.05.003
Conference Contribution - Published proceedings: Abstract
Hally, K. E., Holley, A. S., Kristono, G. A., Harding, S. A., & Larsen, P. D. (2020). Immunoglobulin G levels predicts risk of recurrent adverse cardiovascular events in myocardial infarction patients. Acta Cardiologica, 75(6), 497-502. doi: 10.1080/00015385.2019.1611189
Journal - Research Article
Kristono, G. A., Holley, A. S., Hally, K. E., Brunton-O'Sullivan, M. M., Shi, B., Harding, S. A., & Larsen, P. D. (2020). An IL-6-IL-8 score derived from principal component analysis is predictive of adverse outcome in acute myocardial infarction. Cytokine: X, 2, 100037. doi: 10.1016/j.cytox.2020.100037
Journal - Research Article
Kristono, G. A., Holley, A. S., Harding, S. A., & Larsen, P. D. (2020). White blood cell subtypes as predictors of adverse cardiac events. Coronary Artery Disease, 31(5), 446-450. doi: 10.1097/mca.0000000000000860
Journal - Research Article
Kristono, G. A., Holley, A. S., Lakshman, P., Brunton-O'Sullivan, M. M., Harding, S. A., & Larsen, P. D. (2020). Association between inflammatory cytokines and long-term adverse outcomes in acute coronary syndromes: A systematic review. Heliyon, 6(4), e03704. doi: 10.1016/j.heliyon.2020.e03704
Journal - Research Article
Brunton-O'Sullivan, M., Holley, A., Hally, K., Kristono, G., Bird, G., Harding, S., & Larsen, P. (2020). Examining the relationship between infarct size and circulating extracellular matrix biomarkers over acute and chronic phases of myocardial infarction. Heart, Lung & Circulation, 29(Suppl. 2), (pp. S257-S258). doi: 10.1016/j.hlc.2020.09.495
Conference Contribution - Published proceedings: Abstract
Fahmi, H., Laurie, A., Shepherd, P., Bhoothpur, C., Legget, M., Doughty, R., Holley, A., Larsen, P., & Gladding, P. (2020). Clinical applications of polygenic risk scores in coronary artery disease and familial hypercholesterolaemia. Heart, Lung & Circulation, 29(Suppl. 1), (pp. S5-S6). doi: 10.1016/j.hlc.2020.05.017
Conference Contribution - Published proceedings: Abstract
Holley, A., Harding, S., & Larsen, P. (2020). An analysis on gender disparities in a contemporary acute myocardial infarction (AMI) cohort. Heart, Lung & Circulation, 29(Suppl. 2), (pp. S235). doi: 10.1016/j.hlc.2020.09.447
Conference Contribution - Published proceedings: Abstract
Kristono, G., Holley, A., Hally, K., Brunton-O'Sullivan, M., Harding, S., & Larsen, P. (2020). Trends in inflammatory cytokine levels over acute and chronic phases of myocardial infarction. Heart, Lung & Circulation, 29(Suppl. 2), (pp. S293). doi: 10.1016/j.hlc.2020.09.574
Conference Contribution - Published proceedings: Abstract
Yiu, J., Hally, K., Holley, A., & Larsen, P. (2020). Circulating low-density neutrophils in acute myocardial infarction. Heart, Lung & Circulation, 29(Suppl. 2), (pp. S242). doi: 10.1016/j.hlc.2020.09.464
Conference Contribution - Published proceedings: Abstract
Brunton-O'Sullivan, M., Holley, A., Hally, K., Harding, S., & Larsen, P. (2019). An exploratory factor analysis of circulating extracellular matrix biomarkers in acute myocardial infarction. Heart, Lung & Circulation, 28(Suppl. 1), (pp. S14). doi: 10.1016/j.hlc.2019.05.039
Conference Contribution - Published proceedings: Abstract
Gunawardene, A., Manning, S., Danielson, K., Holley, A., Kristono, G., Shekouh, A., Larsen, P., & Dennett, E. (2019). A combined IL8 and CEA score is an effective marker of metastatic colorectal cancer. ANZ Journal of Surgery, 89(Suppl. 1), (pp. 23). doi: 10.1111/ans.15180
Conference Contribution - Published proceedings: Abstract
Kristono, G., Holley, A., Lakshman, P., Brunton-O'Sullivan, M., Harding, S., & Larsen, P. (2019). A systematic review of inflammatory cytokines as predictors of long-term outcome in acute coronary syndromes. Heart, Lung & Circulation, 28(Suppl. 1), (pp. S19). doi: 10.1016/j.hlc.2019.05.051
Conference Contribution - Published proceedings: Abstract
Kirby, A., Holley, A., Aitken, A., Larsen, P., & Harding, S. (2018). Family history of premature coronary artery disease: Accuracy and predictor of risk. Heart, Lung & Circulation, 27(Suppl. 1), (pp. S26). doi: 10.1016/j.hlc.2018.05.159
Conference Contribution - Published proceedings: Abstract
Kristono, G., Holley, A., Sasse, A., Harding, S., & Larsen, P. (2018). Neutrophil-lymphocyte ratio as a prognostic marker in acute myocardial infarction. Heart, Lung & Circulation, 27(Suppl. 2), (pp. S332-S333). doi: 10.1016/j.hlc.2018.06.645
Conference Contribution - Published proceedings: Abstract
Harding, S. A., Holley, A., Wilkins, B., Fairley, S., Simmonds, M., & Larsen, P. D. (2017). Contemporary antiplatelet therapy in acute coronary syndromes: Are there differences in outcomes and discontinuation between clopidogrel and ticagrelor? Internal Medicine Journal, 47(11), 1298-1305. doi: 10.1111/imj.13595
Journal - Research Article
Holley, A., Pitman, J., Miller, J., Harding, S., & Larsen, P. (2017). Glutathione peroxidase activity and expression levels are significantly increased in acute coronary syndromes. Journal of Investigative Medicine, 65(5), 919-925. doi: 10.1136/jim-2016-000361
Journal - Research Article
Larsen, P. D., Holley, A. S., Sasse, A., Al-Sinan, A., Fairley, S., & Harding, S. A. (2017). Comparison of Multiplate and VerifyNow platelet function tests in predicting clinical outcome in patients with acute coronary syndromes. Thrombosis Research, 152, 14-19. doi: 10.1016/j.thromres.2017.02.006
Journal - Research Article
Matsis, K., Holley, A., Al-Sinan, A., Matsis, P., Larsen, P. D., & Harding, S. A. (2017). Differing clinical characteristics between young and older patients presenting with myocardial infarction. Heart, Lung & Circulation, 26(6), 566-571. doi: 10.1016/j.hlc.2016.09.007
Journal - Research Article
Fake, A., Holley, A., Kirby, A., Sasse, A., Larsen, P., & Harding, S. (2017). Evaluating the ability of bleeding risk scores and platelet reactivity to predict bleeding risk in those presenting with myocardial infarction. Heart, Lung & Circulation, 26(Suppl. 2), (pp. S77). doi: 10.1016/j.hlc.2017.06.078
Conference Contribution - Published proceedings: Abstract
Harding, S., Holley, A., Fairley, S., Wilkins, B., Simmonds, M., & Larsen, P. (2017). Contemporary antiplatelet therapy in acute coronary syndromes: Are there differences in outcomes and complications between clopidogrel and ticagrelor? Heart, Lung & Circulation, 26(Suppl. 2), (pp. S292-S293). doi: 10.1016/j.hlc.2017.06.576
Conference Contribution - Published proceedings: Abstract
Holley, A., Northcott, H., Gladding, P., Harding, S., & Larsen, P. (2017). Significant differences in genetic risk profiles between Maori and European presenting with myocardial infarction. Heart, Lung & Circulation, 26(Suppl. 2), (pp. S307-S308). doi: 10.1016/j.hlc.2017.06.612
Conference Contribution - Published proceedings: Abstract
Holley, A., Wilkins, B., Fairley, S., Ranchord, A., Larsen, P., & Harding, S. (2017). Prior myocardial infarction an independent predictor of adverse cardiovascular events. Heart, Lung & Circulation, 26(Suppl. 2), (pp. S91). doi: 10.1016/j.hlc.2017.06.110
Conference Contribution - Published proceedings: Abstract
Kirby, A., Aitken, A., Simmonds, M., Holley, A., Larsen, P., & Harding, S. (2017). Readmission rates 30 days following myocardial infarction. Heart, Lung & Circulation, 26(Suppl. 1), (pp. S17-S18). doi: 10.1016/j.hlc.2017.05.045
Conference Contribution - Published proceedings: Abstract
Lim, R. Y., Holley, A., Fairley, S., Sasse, A., Larsen, P., & Harding, S. (2017). Ticagrelor use and platelet reactivity in Maori and diabetic patients with acute coronary syndromes. Heart, Lung & Circulation, 26(Suppl. 1), (pp. S5). doi: 10.1016/j.hlc.2017.05.012
Conference Contribution - Published proceedings: Abstract
O'Connor, M., Ranchord, A., Holley, A., Larsen, P., & Harding, S. (2017). What are the clinical and demographic characteristics of patients prescribed less than 12 months of Dual Anti-Platelet Therapy (DAPT) after an Acute Coronary Syndrome (ACS) event? Heart, Lung & Circulation, 26(Suppl. 1), (pp. S20). doi: 10.1016/j.hlc.2017.05.052
Conference Contribution - Published proceedings: Abstract
Wilkins, B., Matsis, P., Simmonds, M., Holley, A., Larsen, P., & Harding, S. (2017). Ticagrelor use and the “treatment risk paradox” in elderly patients with acute coronary syndromes. Heart, Lung & Circulation, 26(Suppl. 1), (pp. S7). doi: 10.1016/j.hlc.2017.05.018
Conference Contribution - Published proceedings: Abstract
Holley, A. S., Miller, J. H., Larsen, P. D., & Harding, S. A. (2016). Relationship between glutathione peroxidase, platelet reactivity, and reactive oxygen species in an acute coronary syndrome population. Annals of Clinical & Laboratory Science, 46(6), 639-644.
Journal - Research Article
Holley, A., Harding, S., Sasse, A., Miller, J., & Larsen, P. (2016). Reduced glutathione peroxidase activity predicts increased cardiovascular risk following an acute coronary syndrome. International Cardiovascular Forum Journal, 6, 61-65. doi: 10.17987/icfj.v6i0.241
Journal - Research Article
Holley, A., Al-Sinan, A., Fairley, S., Simmonds, M., Larsen, P., & Harding, S. (2016). Residual platelet reactivity measured by VerifyNow® is more predictive than Multiplate® of one year outcomes following acute coronary syndromes (ACS). Heart, Lung & Circulation, 25(Suppl. 2), (pp. S62). doi: 10.1016/j.hlc.2016.06.140
Conference Contribution - Published proceedings: Abstract
Holley, A., Matsis, K., Northcott, H., Gladding, P., Harding, S., & Larsen, P. (2016). Genetic risk scoring in a young myocardial infarction (MI) population. Heart, Lung & Circulation, 25(Suppl. 2), (pp. S283-S284). doi: 10.1016/j.hlc.2016.06.664
Conference Contribution - Published proceedings: Abstract
Larsen, P. D., Johnston, L. R., Holley, A., La Flamme, A., Smyth, L., Chua, E. W., Kennedy, M. A., & Harding, S. A. (2015). Prevalence and significance of CYP2C19*2 and CYP2C19*17 alleles in a New Zealand acute coronary syndrome population. Internal Medicine Journal, 45(5), 537-545. doi: 10.1111/imj.12698
Journal - Research Article
Harding, S., Fairley, S., Al-Sinan, A., Holley, A., Howard, C., Matsis, P., Aitken, A., & Larsen, P. (2015). Bleeding, dyspnoea and discontinuation in a real world ACS cohort treated with ticagrelor. Heart, Lung & Circulation, 24(Suppl. 3), (pp. S376). doi: 10.1016/j.hlc.2015.06.615
Conference Contribution - Published proceedings: Abstract
Holley, A., Miller, J., Harding, S., & Larsen, P. (2015). Characterisation of the glutathione peroxidase system in coronary artery disease. Heart, Lung & Circulation, 24(Suppl. 3), (pp. S140). doi: 10.1016/j.hlc.2015.06.064
Conference Contribution - Published proceedings: Abstract
Howard, C., Holley, A., Sasse, A., Harding, S., & Larsen, P. (2015). Is the incidence of high on treatment platelet reactivity (HOTPR) reduced in acute coronary syndrome (ACS) patients treated with ticagrelor? Heart, Lung & Circulation, 24(Suppl. 2), (pp. S100). doi: 10.1016/j.hlc.2015.04.126
Conference Contribution - Published proceedings: Abstract
Hally, K., Johnston, L., Holley, A., Larsen, P., & Harding, S. (2014). Platelet count and platelet function testing in acute coronary syndromes. Heart, Lung & Circulation, 23(Suppl. 1), (pp. e26). doi: 10.1016/j.hlc.2014.04.192
Conference Contribution - Published proceedings: Abstract
Holley, A., Miller, J., Harding, S., & Larsen, P. (2014). Variability and prognostic significance of antioxidant enzymes in acute coronary syndromes (ACS). Heart, Lung & Circulation, 23(Suppl. 1), (pp. e27). doi: 10.1016/j.hlc.2014.04.196
Conference Contribution - Published proceedings: Abstract
Legge, C., Al-Sinan, A., Holley, A., Johnston, L., Larsen, P., & Harding, S. (2014). Does timing of platelet function testing (PFT) contribute to variability in residual on-treatment platelet reactivity in patients with acute coronary syndrome (ACS)? Heart, Lung & Circulation, 23(Suppl. 1), (pp. e31). doi: 10.1016/j.hlc.2014.04.205
Conference Contribution - Published proceedings: Abstract
Plunkett, S., Ranchord, A., Matsis, P., Holley, A., Larsen, P., & Harding, S. (2014). "Normal" coronary arteries in the setting of acute coronary syndromw: Frequency and outcomes. Heart, Lung & Circulation, 23(Suppl. 1), (pp. e36-e37). doi: 10.1016/j.hlc.2014.04.222
Conference Contribution - Published proceedings: Abstract
Holley, A. S., Johnston, L. R., Harding, S. A., & Larsen, P. D. (2013). Reduced glutathione peroxidase (GPx) activity in acute coronary syndrome (ACS) is correlated with high levels of reactive oxygen species (ROS). Heart, Lung & Circulation, 22(7), (pp. 560-561). doi: 10.1016/j.hlc.2013.04.033
Conference Contribution - Published proceedings: Abstract
Holley, A., Johnston, L., Harding, S., & Larsen, P. (2013). High levels of reactive oxygen species (ROS) are associated with reduced glutathione peroxidase (GPx) activity in acute coronary syndrome (ACS). Heart, Lung & Circulation, 22(Suppl. 1), (pp. S41). doi: 10.1016/j.hlc.2013.05.098
Conference Contribution - Published proceedings: Abstract
Johnston, L., Smyth, L., Kennedy, M., Holley, A., Sasse, A., La Flamme, A., Larsen, P., & Harding, S. (2013). High on treatment platelet reactivity is associated with a higher risk of MACE in ACS patients: Diabetes and loss of function genotype the driving forces. Heart, Lung & Circulation, 22(7), (pp. 561). doi: 10.1016/j.hlc.2013.04.035
Conference Contribution - Published proceedings: Abstract
Chen-Xu, M., Johnston, L., Holley, A., Sasse, A., Larsen, P. D., & Harding, S. A. (2012). Comparison of the Multiplate and VerifyNow assays for monitoring residual platelet reactivity after clopidogrel. Heart, Lung & Circulation, 21(8), (pp. 483-484). doi: 10.1016/j.hlc.2012.03.025
Conference Contribution - Published proceedings: Abstract
Holley, A. S., Johnston, L. R., Chen-Xu, M., Sasse, A., Larsen, P. D., & Harding, S. A. (2012). Plasma antioxidant enzyme levels in acute coronary syndrome. Heart, Lung & Circulation, 21(8), (pp. 490). doi: 10.1016/j.hlc.2012.03.040
Conference Contribution - Published proceedings: Abstract
Johnston, L. R., Holley, A. S., Chen-Xu, M., La Flamme, A. C., Larsen, D., & Harding, S. A. (2012). High residual platelet reactivity on aspirin in a New Zealand ACS population. Heart, Lung & Circulation, 21(8), (pp. 491-492). doi: 10.1016/j.hlc.2012.03.043
Conference Contribution - Published proceedings: Abstract
Johnston, L., Holley, A., Chen-Xu, M., Larsen, P., & Harding, S. (2012). Significant disagreement between the VerifyNow and Multiplate assays in the classification of high platelet reactivity on clopidogrel. Heart, Lung & Circulation, 21(Suppl. 1), (pp. S174-S175). doi: 10.1016/j.hlc.2012.05.434
Conference Contribution - Published proceedings: Abstract
