Our experienced team can assist with project design, provide advice for sample / library preparation and ensure that the chosen technology can meet specific project and data requirements.
The Otago Genomics Facility (OGF) is an Illumina Propel-certified service provider for Illumina HiSeq 2500 and MiSeq sequencing platforms.
Illumina sequencing enables a range of DNA and RNA based application including genome and targeted capture sequencing, mutation discovery, transcriptome profiling and the identification of epigenetic changes.
The HiSeq 2500 sequencing system offers the flexibility of Rapid or High Output sequencing. Integration of a MiSeq QC in the sequencing process has optimised library performance providing tighter control of multiplexing within HiSeq sequencing lanes.
The MiSeq sequencing system offers longer read lengths with high quality data with short run times.
We currently offer:
- Whole genome sequencing
- Metagenomics and amplicon sequencing
Nanostring nCounter Analysis System
The Otago Genomics Facility is a Nanostring nCounter Analysis System core facility.
The NanoString nCounter Analysis System offers an alternative to qPCR, and is a cost-effective way to easily profile the expression of hundreds of genes, proteins, miRNA, or copy number variants simultaneously with high sensitivity and precision, facilitating a wide variety of basic research and translational medicine applications, including biomarker discovery and validation.
Nanostring nCounter also provides a validation tool for next generation sequencing data. The robust nature of the amplification-free chemistry allows researchers to measure subtle changes from small and degraded samples such as clinical tissue biopsies.
We currently offer:
- Gene expression analysis: Custom and off-the-shelf Codesets
- Single cell gene expression analysis
- miRNA expression analysis
- miRGE (miRNA & mRNA analysis)
- Copy Number Variation analysis
Staff welcome enquiries and encourage you to contact them early in the design phase of your project so that they can provide the best advice to achieve high quality research design and data.