Dr Torsten Kleffmann

2026

Journal - Research Article

Ward, V. K., McKenzie-Goldsmith, G. M., Edwards, M. J., Young, V. L., Kleffmann, T., Stubbing, L. A., … Brimble, M. A. (2026). The MNV-1 protease–polymerase precursor cleaves a novel site in the NS1-2 protein. Journal of General Virology, 107, 002263. doi: 10.1099/jgv.0.002263

Kinaston, R. L., Keith, S., Hudson, B., Geber, J., Kleffmann, T., Stirling, C., Reid, M., Barr, D., … & Waka Kotahi Tangata Whenua Working Group. (2026). Horticultural intensification and plant-based diets of 18th century CE Waikato Māori in Aotearoa New Zealand. Nature Communications, 17, 3040. doi: 10.1038/s41467-026-70128-5

Paterson, E. S. J., Scheck, S., Kleffmann, T., Gill, R., Bedford, N., McDowell, S., Girling, J. E., & Henry, C. E. (2026). Proteomic analysis of cervicovaginal fluid for diagnostic endometriosis biomarker discovery. Proteomics, 20, e70044. doi: 10.1002/prca.70044

2025

Chapter in Book - Research

Kumar, A., Peppercorn, K., & Kleffmann, T. (2025). Quantitative proteomics on immune cells of ME/CFS patients using SWATH-MS. In W. P. Tate & K. Peppercorn (Eds.), Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): Methods and protocols: Methods in molecular biology (Vol. 2920). (pp. 113-140). New York, NY: Humana Press. doi: 10.1007/978-1-0716-4498-0_8

Journal - Research Article

King, C. L., Snoddy, A. M., Kleffmann, T., Petchey, P., & Buckley, H. R. (2025). Osteological sex estimation versus proteomic sex “determination”: What effect do our techniques have on the way we view the people of the past? American Journal of Biological Anthropology, 188(3), e70170. doi: 10.1002/ajpa.70170

Broomfield, G. R., Kleffmann, T., Saul, D. J., & Wilbanks, S. M. (2025). Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position. FEBS Letters. Advance online publication. doi: 10.1002/1873-3468.70123

Reddington, C. J., Walsh, A. R., Kleffmann, T., Göbl, C., & Mace, P. D. (2025). Unconventional structure and function of PHD domains from additional sex combs-like proteins. FEBS Journal. Advance online publication. doi: 10.1111/febs.70206

Willis, A., Pradier, B., Rogers, L., Halcrow, S., Kleffmann, T., Cali, J., … Pryce, T. O. (2025). Living in the middle of the edge: An insight into ancient subsistence practices in Myanmar. Archaeological & Anthropological Sciences, 17, 150. doi: 10.1007/s12520-025-02265-9

2024

Journal - Research Article

Saseendran Nair, S., Kleffmann, T., Smith, B., Morris, V., Göbl, C., Pletzer, D., & Fellner, M. (2024). Comparative lipidomics profiles of planktonic and biofilms of methicillin-resistant and -susceptible Staphylococcus aureus. Analytical Biochemistry, 698, 115746. doi: 10.1016/j.ab.2024.115746

Rogers, L. M., Halcrow, S. E., Kleffmann, T., & King, C. L. (2024). Comparing Walker's (2008) skull trait sex estimation standard to proteomic sex estimation for a group of South Asian individuals. Forensic Science International: Synergy, 8, 100450. doi: 10.1016/j.fsisyn.2023.100450

Hawkins, S., Zetika, G. A., Kinaston, R., Firmando, Y. R., Sari, D. M., Suniarti, Y., … Stirling, C., Reid, M., Barr, D., Kleffmann, T., Kumar, A., … O'Connor, S. R. (2024). Earliest known funerary rites in Wallacea after the last glacial maximum. Scientific Reports, 14, 282. doi: 10.1038/s41598-023-50294-y

Major, G., Simcock, J., Kumar, A., Kleffmann, T., Woodfield, T. B. F., & Lim, K. S. (2024). Comprehensive matrisome profiling of human adipose tissue for soft tissue reconstruction. Advanced Biology, 8, 2300448. doi: 10.1002/adbi.202300448

Sudron, E. L., Kinaston, R. L., Cawte, H., Kleffmann, T., Kumar, A., Kramer, R., Stirling, C., Reid, M., Barr, D., … King, K., & Halcrow, S. E. (2024). Extracting the truth through chemical analyses: Early life histories of Victorian-era dental patients in Aotearoa New Zealand. American Journal of Biological Anthropology, 183, 141-156. doi: 10.1002/ajpa.24863

2023

Journal - Research Article

Peppercorn, K., Edgar, C. D., Kleffmann, T., & Tate, W. P. (2023). A pilot study on the immune cell proteome of long COVID patients shows changes to physiological pathways similar to those in myalgic encephalomyelitis/chronic fatigue syndrome. Scientific Reports, 13(1), 22068. doi: 10.1038/s41598-023-49402-9

Walker, M. O. M., Peppercorn, K., Kleffmann, T., Edgar, C. D., & Tate, W. P. (2023). An understanding of the immune dysfunction in susceptible people who develop the post-viral fatigue syndromes Myalgic Encephalomyelitis/Chronic Fatigue Syndrome and Long COVID. Medical Research Archives, 11(7). doi: 10.18103/mra.v11i7.1.4083

Winterbourn, C. C., Peskin, A., Kleffmann, T., Radi, R., & Pace, P. E. (2023). Carbon dioxide/bicarbonate is required for sensitive inactivation of mammalian glyceraldehyde-3-phosphate dehydrogenase by hydrogen peroxide. PNAS, 120(18), e2221047120. doi: 10.1073/pnas.2221047120

Peppercorn, K., Kleffmann, T., Hughes, S. M., & Tate, W. P. (2023). Secreted amyloid precursor protein alpha (sAPPα) regulates the cellular proteome and secretome of mouse primary astrocytes. International Journal of Molecular Sciences, 24, 7165. doi: 10.3390/ijms24087165

Templeton, E. M., Pilbrow, A. P., Kleffmann, T., Pickering, J. W., Rademaker, M. T., Scott, N. J. A., Ellmers, L. J., Charles, C. J., Endre, Z. H., Richards, A. M., Cameron, V. A., & Lassé, M. (2023). Comparison of SPEED, S-Trap, and in-solution-based sample preparation methods for mass spectrometry in kidney tissue and plasma. International Journal of Molecular Sciences, 24(7), 6290. doi: 10.3390/ijms24076290

Paget, B. W., Kleffmann, T., Whiteman, K. E., Thomas, M. F., & McMahon, C. D. (2023). Quantitative comparison of manuka and clover honey proteomes with royal jelly. PLoS ONE, 18(2), e0272898. doi: 10.1371/journal.pone.0272898

Conference Contribution - Published proceedings: Abstract

Broomfield, G., Kumar, A., Saul, D., Stanton, J.-A., Kleffmann, T., & Wilbanks, S. M. (2023). Cleavage preference of the thermophilic metalloprotease, EA1. Proceedings of the New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) 50th Anniversary Conference. Retrieved from https://www.nzsbmb.org/conference

Lu, A., Kleffmann, T., Ledgerwood, E. C., & Day, C. L. (2023). Characterisation of the role of RNF125 in RIG-I antiviral signalling. Proceedings of the New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) 50th Anniversary Conference. Retrieved from https://www.nzsbmb.org/conference

Reddington, C., Walsh, A., Kleffmann, T., Goebl, C., Hinde, E., Day, C., & Mace, P. (2023). Redefining the ASXL PHD domain as a DNA damage recognition factor. Proceedings of the New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) 50th Anniversary Conference. Retrieved from https://www.nzsbmb.org/conference

Conference Contribution - Poster Presentation (not in published proceedings)

Bland, A. R., Kleffmann, T., & Ashton, J. C. (2023, August). Using next generation mass spectrometry technologies to examine protein targets of the ALK+ lung cancer drug, Alectinib. Poster session presented at the 17th New Zealand Medical Sciences Congress (MedSci), Queenstown, New Zealand.

Conference Contribution - Verbal presentation and other Conference outputs

Reddington, C. J., Kleffmann, T., Day, C. L., & Mace, P. D. (2023, August). Redefining the ASXL PHD domain as a DNA damage recognition factor. Verbal presentation at the 267th Otago Medical School Research Society (OMSRS) Meeting: PhD Student Speaker Awards, Dunedin, New Zealand.

2022

Journal - Research Article

Peppercorn, K., Kleffmann, T., Jones, O., Hughes, S., & Tate, W. (2022). Secreted amyloid precursor protein alpha, a neuroprotective protein in the brain has widespread effects on the transcriptome and proteome of human inducible pluripotent stem cell-derived glutamatergic neurons related to memory mechanisms. Frontiers in Neuroscience, 16, 858524. doi: 10.3389/fnins.2022.858524

Warring, S. L., Malone, L. M., Jayaraman, J., Easingwood, R. A., Rigano, L. A., Frampton, R. A., … Kleffmann, T., … Bostina, M., & Fineran, P. C. (2022). A lipopolysaccharide-dependent phage infects a pseudomonad phytopathogen and can evolve to evade phage resistance. Environmental Microbiology, 24(10), 4834-4852. doi: 10.1111/1462-2920.16106

Handley, T. N. G., Kleffmann, T., & Butler, M. I. (2022). Development of ULYSSIS, a tool for the biosynthesis of cyclotides and cyclic knottins. International Journal of Peptide Research & Therapeutics, 28, 21. doi: 10.1007/s10989-021-10336-3

Templeton, E. M., Lassé, M., Kleffmann, T., Ellmers, L. J., Palmer, S. C., Davidson, T., Scott, N. J. A., Pickering, J. W., Charles, C. J., Endre, Z. H., Cameron, V. A., Richards, A. M., Rademaker, M. T., & Pilbrow, A. P. (2022). Identifying candidate protein markers of acute kidney injury in acute decompensated heart failure. International Journal of Molecular Sciences, 23, 1009. doi: 10.3390/ijms23021009

Conference Contribution - Published proceedings: Abstract

Faiz, M., Kalev-Zylinska, M., Kleffmann, T., Day, R., & Ledgerwood, E. C. (2022). Elevated Ca²⁺ flux plays a vital role in type I calreticulin mutations. In L. Wilson, H. Harcombe, P. Jayakaran, L. Burga, J. Antony, K. Morgaine, M. Garelja, A. Middleton, M. Anwar & T. Milne (Eds.), Proceedings of the 263rd Otago Medical School Research Society (OMSRS) Meeting: PhD Student Speaker Awards. Dunedin, New Zealand: OMSRS. Retrieved from https://ourarchive.otago.ac.nz/handle/10523/12839

Peppercorn, K., Kleffmann, T., Jones, O., Hughes, S., & Tate, W. (2022). Secreted amyloid precursor protein alpha has widespread effects on the transcriptome and proteome of human neurons related to memory mechanisms. In K. Horne (Ed.), Proceedings of the 38th International Australasian Winter Conference on Brain Research (AWCBR). (pp. 64). Retrieved from https://www.queenstownresearchweek.org

Templeton, E., Lassé, M., Cameron, V., Pickering, J., Kleffmann, T., Hertzberg, D., Frampton, C., Ellmers, L., Palmer, S., … Scott, N., Charles, C., … Richards, M., … Troughton, R., Rademaker, M., & Pilbrow, A. (2022). Harnessing the power of 'Omics': Discovering novel microRNA and protein biomarkers of acute kidney injury in acute heart failure. Heart, Lung & Circulation, 31(Suppl. 2), (pp. S23-S24). doi: 10.1016/j.hlc.2022.05.009

Conference Contribution - Verbal presentation and other Conference outputs

Faiz, M., Kalev-Zylinska, M., Kleffmann, T., Day, R., & Ledgerwood, E. C. (2022, September). Elevated calcium flux plays a vital role in dual-guide CRISPR/Cas9 generated type I calreticulin mutant cells. Verbal presentation at the Postgraduate & Early Career Researchers Committee (PERC) & Division of Health Sciences Early & Mid-Career Researcher (EMCR) Joint Research Symposium, Dunedin, New Zealand.

Faiz, M., Kalev-Zylinska, M., Kleffmann, T., Day, R., & Ledgerwood, E. C. (2022, August). Elevated endoplasmic reticulum calcium flux plays a vital role in dual-guide CRISPR/Cas9 generated type I calreticulin mutant cells. Verbal presentation at the CRISPR Technologies Satellite Meeting: Queenstown Research Week, Queenstown, New Zealand.

2021

Journal - Research Article

Burgess, A. E., Kleffmann, T., & Mace, P. D. (2021). Oncogenic truncations of ASXL1 enhance a motif for BRD4 ET-domain binding. Journal of Molecular Biology, 433, 167242. doi: 10.1016/j.jmb.2021.167242

Ahmed, F., Kleffmann, T., & Husain, M. (2021). Acetylation, methylation and allysine modification profile of viral and host proteins during influenza A virus infection. Viruses, 13(7), 1415. doi: 10.3390/v13071415

Philem, P., Kleffmann, T., Gai, S., Hawkins, B. C., Wilbanks, S. M., & Lamont, I. L. (2021). Identification of active site residues of the siderophore synthesis enzyme PvdF and evidence for interaction of PvdF with a substrate-providing enzyme. International Journal of Molecular Sciences, 22(4), 2211. doi: 10.3390/ijms22042211

Lassé, M., Pilbrow, A. P., Kleffmann, T., Andersson Överström, E., von Zychlinski, A., Frampton, C. M. A., … Troughton, R. W., Lewis, L. K., Prickett, T. C. R., Pemberton, C. J., Richards, A. M., & Cameron, V. A. (2021). Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals. Scientific Reports, 11(1), 4605. doi: 10.1038/s41598-021-84046-7

Conference Contribution - Published proceedings: Abstract

Templeton, E., Lassé, M., Kleffmann, T., Ellmers, L. J., Palmer, S. C., Davidson, T., Scott, N. J., Pickering, J. W., Charles, C. J., Endre, Z. H., Cameron, V. A., & Richards, A. M. (2021). Identifying candidate protein markers of acute kidney injury in acute decompensated heart failure. Heart, Lung & Circulation, 30(Suppl. 2), (pp. S66). doi: 10.1016/j.hlc.2021.05.020

Lim, C. S., Wardell, S., Kleffmann, T., Weinstein, B., Roy, S., & Brown, C. (2021). The evolution and function of introns. Proceedings of the 16th Congress of the Federation of Asian & Oceanic Biochemists & Molecular Biologists (FAOBMB). (pp. 161). Retrieved from https://www.faobmb2021.org

2020

Journal - Research Article

Johnson, S. L., Borziak, K., Kleffmann, T., Rosengrave, P., Dorus, S., & Gemmell, N. J. (2020). Ovarian fluid proteome variation associates with sperm swimming speed in an externally fertilising fish. Journal of Evolutionary Biology, 33, 1783-1794. doi: 10.1111/jeb.13717

Murphy, E. L., Singh, K. V., Avila, B., Kleffmann, T., Gregory, S. T., Murray, B. E., Krause, K. L., … Jogl, G. (2020). Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis. Scientific Reports, 10, 16301. doi: 10.1038/s41598-020-73199-6

Sweetman, E., Kleffmann, T., Edgar, C., de Lange, M., Vallings, R., & Tate, W. (2020). A SWATH-MS analysis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome peripheral blood mononuclear cell proteomes reveals mitochondrial dysfunction. Journal of Translational Medicine, 18, 365. doi: 10.1186/s12967-020-02533-3

Mehta, S., Algie, M., Al-Jabry, T., McKinney, C., Kannan, S., Verma, C. S., … Parker, K., Henderson, L., Gould, M. L., Bhatia, P., Harfoot, R., … Kleffmann, T., … Woolley, A. G., … Braithwaite, A. (2020). Critical role for cold shock protein YB-1 in cytokinesis. Cancers, 12, 2473. doi: 10.3390/cancers12092473

Handley, T. N. G., Tan, H.-X., Rutledge, M. T., Brewitz, H. H., Tyndall, J. D. A., Kleffmann, T., Butler, M. I., Poulter, R. T. M., & Wilbanks, S. M. (2020). Engineered biosynthesis of cyclotides. New Zealand Journal of Botany, 58(4), 358-377. doi: 10.1080/0028825X.2020.1791914

Mehta, S., McKinney, C., Algie, M., Verma, C. S., Kannan, S., Harfoot, R., … Gould, M. L., Parker, K., … Cunliffe, H. E., … Kleffmann, T., Braithwaite, A. W., & Woolley, A. G. (2020). Dephosphorylation of YB-1 is required for nuclear localisation during G₂ phase of the cell cycle. Cancers, 12(2), 315. doi: 10.3390/cancers12020315

Conference Contribution - Published proceedings: Abstract

Lal, N., Cornwall, J., Slatter, T., Kleffmann, T., & Sheard, P. (2020). Proteomic differences between young, elderly, and exercised-elderly murine soleus muscles and their correlations with deficits in force production. Australasian Journal on Ageing, 39(Suppl. 2), (pp. 18). doi: 10.1111/ajag.12808

2019

Journal - Research Article

Herbison, R., Evans, S., Doherty, J.-F., Algie, M., Kleffmann, T., & Poulin, R. (2019). A molecular war: Convergent and ontogenetic evidence for adaptive host manipulation in related parasites infecting divergent hosts. Proceedings of the Royal Society B, 286(1915), 20191827. doi: 10.1098/rspb.2019.1827

Crake, R. L. I., Phillips, E., Kleffmann, T., & Currie, M. J. (2019). Co-culture with human breast adipocytes differentially regulates protein abundance in breast cancer cells. Cancer Geonomics & Proteomics, 16(5), 319-332. doi: 10.21873/cgp.20137

Conference Contribution - Published proceedings: Abstract

Kleffmann, T., Tate, W., & Hughes, S. (2019). Does secreted amyloid precursor protein alpha affect the global proteome of astrocytes? Proceedings of the 39th Australasian Neuroscience Society (ANS) Annual Scientific Meeting. 13247. Retrieved from https://www.ans.org.au

Budhwani, M., McLellan, A., Kleffmann, T., & Hibma, M. (2019). Extracellular vesicles released from human papillomavirus type 16 E7 keratinocytes suppress antigen presenting cell activation and antigen-specific T cell proliferation. European Journal of Immunology, 49(Suppl. 2), (pp. 7-8). doi: 10.1002/eji.201970200

2018

Journal - Research Article

Rodger, E. J., Porteous, C. M., Jones, G. T., Legge, M., Kleffmann, T., & McCormick, S. P. A. (2018). Proteomic analysis of liver from lipoprotein(a) transgenic mice shows an oxidative stress and lipid export response. BioMed Research International, 2018, 4963942. doi: 10.1155/2018/4963942

Parakra, R. D., Kleffmann, T., Jameson, G. N. L., & Ledgerwood, E. C. (2018). The proportion of Met80-sulfoxide dictates peroxidase activity of human cytochrome c. Dalton Transactions, 47, 9128. doi: 10.1039/c8dt02185f

Lim, C. S., Wardell, S. J. T., Kleffmann, T., & Brown, C. M. (2018). The exon-intron gene structure upstream of the initiation codon predicts translation efficiency. Nucleic Acids Research, 46(9), 4575-4591. doi: 10.1093/nar/gky282

González-Miró, M., Rodríguez-Noda, L. M., Fariñas-Medina, M., Cedré-Marrero, B., Madariaga-Zarza, S., Zayas-Vignier, C., … Kleffmann, T., … Rehm, B. H. A. (2018). Bioengineered polyester beads co-displaying protein and carbohydrate-based antigens induce protective immunity against bacterial infection. Scientific Reports, 8, 1888. doi: 10.1038/s41598-018-20205-7

Mortuza, R., Aung, H. L., Taiaroa, G., Opel-Reading, H. K., Kleffmann, T., Cook, G. M., & Krause, K. L. (2018). Overexpression of a newly identified d-amino acid transaminase in Mycobacterium smegmatis complements glutamate racemase deletion. Molecular Microbiology, 107(2), 198-213. doi: 10.1111/mmi.13877

Conference Contribution - Published proceedings: Abstract

Malone, L. M., Warnecke, C., Kleffmann, T., Morgan, X. C., & Fineran, P. C. (2018). The activity of type I-E, I-F and III-A CRIPR-CAS systems during phage infection. Proceedings of the New Zealand Microbiological Society (NZMS) New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) Joint Annual Conference: Microbes & Molecules. (pp. 106). Retrieved from https://www.nzmsconference.org.nz

Krause, K. L., Jogl, G., Khayat, R., Murphy, E. L., Murray, B. E., Singh, K., & Kleffmann, T. (2018). Exploring the enterococcal ribosome structure as a template for antibiotic development. Proceedings of the New Zealand Microbiological Society (NZMS) New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) Joint Annual Conference: Microbes & Molecules. (pp. 61-62). Retrieved from https://www.nzmsconference.org.nz

Phillips, E., Kleffmann, T., Morrin, H., Robinson, B., & Currie, M. (2018). Differential secretome analysis of cancer-associated adipocytes (CAA) and mature adipocytes to identify adipocyte-driven micro-environmental regulators of breast cancer progression. ESMO Open, 3(Suppl. 2), (pp. A348). doi: 10.1136/esmoopen-2018-EACR25.820

McCormick, S., Porteous, C., Sharma, M., Jones, G., Legge, M., Kleffmann, T., & Rodger, E. (2018). Proteomic analysis of liver from human Lipoprotein(a) transgenic mice shows an oxidative stress and lipid export response. Atherosclerosis Supplements, 32, (pp. 4). doi: 10.1016/j.atherosclerosissup.2018.04.013

Parakra, R., Kleffmann, T., Jameson, G., & Ledgerwood, E. (2018). Investigating peroxidase activity and cardiolipin oxidation in human cytochrome c variants. Free Radical Biology & Medicine, 120(Suppl. 1), (pp. S99). doi: 10.1016/j.freeradbiomed.2018.04.327

Conference Contribution - Poster Presentation (not in published proceedings)

Thomson, J., Algie, M., Kleffmann, T., & Lamont, I. (2018, November). Determining the molecular effects of 5-fluorocytosine on the pathogenicity of Pseudomonas aeruginosa. Poster session presented at the Proceedings of the New Zealand Microbiological Society (NZMS) New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) Joint Annual Conference: Microbes & Molecules, Dunedin, New Zealand.

Crake, R., Kleffmann, T., Morrin, H., Strother, M., Robinson, B., Phillips, E., & Currie, M. (2018, August). Cancer-associated adipocytes alter protein expression profiles of human breast cancer cells in vitro. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Mehta, S., Algie, M., Al Jabri, T., McKinney, C., Verma, C., Weini, M., … Henderson, L., Gould, M., Parker, K., Bhatia, P., Harfoot, R., Kleffmann, T., … Woolley, A., & Braithwaite, A. (2018, August). RSK mediated YB-1 phosphorylation: A key event required for completion of cytokinesis. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

2017

Conference Contribution - Published proceedings: Abstract

Munir, J., Kleffmann, T., & McCormick, S. (2017). Proteomic analysis of 4-phenylbutyrate treated HepG2 cells stably expressing ATP-binding Cassette Transporter A1 (ABCA1) mutants. Proceedings of the University of Otago Student Research Symposium: Te Wānaka Rakahau: Ākoka. (pp. 60). Retrieved from http://www.otago.ac.nz/graduate-research/scholarships/otago643219.html

Conference Contribution - Poster Presentation (not in published proceedings)

Munir, J., Kleffmann, T., & McCormick, S. P. (2017, September). Proteomic analysis of 4-phenylbutyrate treated HepG2 cells stably expressing ATP-binding cassette transporter A1 (ABCA1) mutants. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

2016

Journal - Research Article

Wei, J., Lau, S. Y., Blenkiron, C., Chen, Q., James, J. L., Kleffmann, T., … Chamley, L. W. (2016). Trophoblastic debris modifies endothelial cell transcriptome in vitro: A mechanism by which fetal cells might control maternal responses to pregnancy. Scientific Reports, 6, 30632. doi: 10.1038/srep30632

Muhsin-Sharafaldine, M.-R., Saunderson, S. C., Dunn, A. C., Faed, J. M., Kleffmann, T., & McLellan, A. D. (2016). Procoagulant and immunogenic properties of melanoma exosomes, microvesicles and apoptotic vesicles. Oncotarget, 7(35), 56279-56294. doi: 10.18632/oncotarget.10783

Tong, M., Kleffmann, T., Pradhan, S., Johansson, C. L., DeSousa, J., Stone, P. R., … Chamley, L. W. (2016). Proteomic characterization of macro-, micro- and nano-extracellular vesicles derived from the same first trimester placenta: Relevance for feto-maternal communication. Human Reproduction, 31(4), 687-699. doi: 10.1093/humrep/dew004

Conference Contribution - Verbal presentation and other Conference outputs

Tong, B., Watson, B. N. J., Frampton, R. A., Young, V. L., Taylor, C., Easingwood, R. A., Pitman, A. R., Kleffmann, T., … Fineran, P. C., & Bostina, M. (2016, November). Cryo-electron microscopy structure and proteome of phages infecting plant pathogenic bacteria. Verbal presentation at the Joint Meeting of the New Zealand Microbiological Society (NZMS) and the New Zealand Society for Biochemistry and Molecular Biology (NZSBMB), Christchurch, New Zealand.

Muhsin-Sharafaldine, M.-R., Saunderson, S. C., Dunn, A. C., Faed, J. M., Kleffmann, T., & McLellan, A. D. (2016, August-September). Procoagulant and immunogenic properties of tumour exosomes, microvesicles and apoptotic vesicles. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.

2015

Journal - Research Article

Pantham, P., Viall, C. A., Chen, Q., Kleffmann, T., Print, C. G., & Chamley, L. W. (2015). Antiphospholipid antibodies bind syncytiotrophoblast mitochondria and alter the proteome of extruded syncytial nuclear aggregates. Placenta, 36, 1463-1473. doi: 10.1016/j.placenta.2015.10.006

Cardno, T. S., Shimaki, Y., Sleebs, B. E., Lackovic, K., Parisot, J. P., Moss, R. M., Crowe-McAuliffe, C., Mathew, S. F., Edgar, C. D., Kleffmann, T., & Tate, W. P. (2015). HIV-1 and human PEG10 frameshift elements are functionally distinct and distinguished by novel small molecule modulators. PLoS ONE, 10(10), e0139036. doi: 10.1371/journal.pone.0139036

Tchesnokov, E. P., Fellner, M., Siakkou, E., Kleffmann, T., Martin, L. W., Aloi, S., Lamont, I. L., Wilbanks, S. M., & Jameson, G. N. L. (2015). The cysteine dioxygenase homologue from Pseudomonas aeruginosa is a 3-mercaptopropionate dioxygenase. Journal of Biological Chemistry, 290(40), 24424-24437. doi: 10.1074/jbc.M114.635672

Kohn, Y. Y., Symonds, J. E., Kleffmann, T., Nakagawa, S., Lagisz, M., & Lokman, P. M. (2015). Proteomic analysis of early-stage embryos: Implications for egg quality in hapuku (Polyprion oxygeneios). Fish Physiology & Biochemistry, 41(6), 1403-1417. doi: 10.1007/s10695-015-0095-0

Frampton, R. A., Lopez Acedo, E., Young, V. L., Chen, D., Tong, B., Taylor, C., Easingwood, R. A., … Kleffmann, T., Bostina, M., & Fineran, P. C. (2015). Genome, proteome and structure of a T7-like bacteriophage of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae. Viruses, 7(7), 3361-3379. doi: 10.3390/v7072776

Guy, P. L., Easingwood, R., Carne, D. L., & Kleffmann, T. (2015). Identification of two plant viruses using partial purification and mass spectrometry. Australasian Plant Disease Notes, 10, 11. doi: 10.1007/s13314-015-0162-5

von Zychlinski, A., & Kleffmann, T. (2015). Dissecting the proteome of lipoproteins: New biomarkers for cardiovascular diseases? Translational Proteomics, 7, 30-39. doi: 10.1016/j.trprot.2014.12.001

Ramsay, J. P., Tester, L. G. L., Major, A. S., Sullivan, J. T., Edgar, C. D., Kleffmann, T., … Tate, W. P., … Ronson, C. W. (2015). Ribosomal frameshifting and dual-target antiactivation restrict quorum-sensing–activated transfer of a mobile genetic element. PNAS, 112(3), 4104-4109. doi: 10.1073/pnas.1501574112

Conference Contribution - Poster Presentation (not in published proceedings)

Lee, W. J., Fleming, S. B., Kleffmann, T., & Mercer, A. A. (2015, August-September). Identifying the interacting partner(s) of orf virus ankyrin repeat proteins. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

2014

Journal - Research Article

Johnson, S. L., Villarroel, M., Rosengrave, P., Carne, A., Kleffmann, T., Lokman, P. M., & Gemmell, N. J. (2014). Proteomic analysis of Chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. PLoS ONE, 9(8), e104155. doi: 10.1371/journal.pone.0104155

Marchalant, Y., Brownjohn, P. W., Bonnet, A., Kleffmann, T., & Ashton, J. C. (2014). Validating antibodies to the cannabinoid CB2 receptor: Antibody sensitivity is not evidence of antibody specificity. Journal of Histochemistry & Cytochemistry, 62(6), 395-404. doi: 10.1369/0022155414530995

Pemberton, C. J., Siriwardena, M., Kleffmann, T., & Richards, A. M. (2014). C-type natriuretic peptide (CNP) signal peptide fragments are present in the human circulation. Biochemical & Biophysical Research Communications, 449, 301-306. doi: 10.1016/j.bbrc.2014.05.020

von Zychilinski, A., Williams, M., McCormick, S., & Kleffmann, T. (2014). Absolute quantification of apolipoproteins and associated proteins on human plasma lipoproteins. Journal of Proteomics, 106, 181-190. doi: 10.1016/j.jprot.2014.04.030

2013

Journal - Research Article

Souness, R. J., Kleffmann, T., Tchesnokov, E. P., Wilbanks, S. M., Jameson, G. B., & Jameson, G. N. L. (2013). Mechanistic implications of persulfenate and persulfide binding in the active site of cysteine dioxygenase. Biochemistry, 52(43), 7606-7617. doi: 10.1021/bi400661a

Pemberton, C. J., & Kleffmann, T. (2013). Circulating signal peptides: More than just a bus ticket? Cardiovascular Endocrinology, 2(3), 41-45. doi: 10.1097/XCE.0b013e328362e44e

Nakatani, Y., Kleffmann, T., Linke, K., Condon, S. M., Hinds, M. G., & Day, C. L. (2013). Regulation of ubiquitin transfer by XIAP, a dimeric RING E3 ligase. Biochemical Journal, 450(3), 629-638. doi: 10.1042/BJ20121702

Kumar, V., Kleffmann, T., Hampton, M. B., Cannell, M. B., & Winterbourn, C. C. (2013). Redox proteomics of thiol proteins in mouse heart during ischemia/reperfusion using ICAT reagents and mass spectrometry. Free Radical Biology & Medicine, 58, 109-117. doi: 10.1016/j.freeradbiomed.2013.01.021

Conference Contribution - Verbal presentation and other Conference outputs

von Zychilinski, A., Williams, M., Kleffmann, T., & McCormick, S. (2013, August). What is really on our lipoproteins? Quantitative information about the protein makeup of the major lipoprotein classes. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

2012

Journal - Research Article

Rodger, E. J., Suetani, R. J., Jones, G. T., Kleffmann, T., Carne, A., Legge, M., & McCormick, S. P. A. (2012). Proteomic analysis of aortae from human lipoprotein(a) transgenic mice shows an early metabolic response independent of atherosclerosis. PLoS ONE, 7(1), e30383. doi: 10.1371/journal.pone.0030383

Pemberton, C. J., Siriwardena, M., Kleffmann, T., Ruygrok, P., Palmer, S. C., Yandle, T. G., & Richards, A. M. (2012). First identification of circulating Prepro-A-Type natriuretic peptide (PreproANP) signal peptide fragments in humans: Initial assessment as cardiovascular biomarkers. Clinical Chemistry, 58(4), 757-767. doi: 10.1373/clinchem.2011.176990

Conference Contribution - Published proceedings: Abstract

Morley, T. D., Redpath, G. M. I., Kleffmann, T., von Zychilinski, A., Williams, M. J. A., & McCormick, S. P. A. (2012). Characterisation of the low density lipoprotein receptor binding properties of lipoproteins from a familial hypoalphalipoproteinemia subject. Proceedings of the Medical Sciences Congress (MedSci). (pp. 68). Retrieved from http://www.medscinz.co.nz/QRW2012%20MedSci%20Abstracts.pdf

Byers, M. S., Siriwardena, M., Ruygrok, P., Yandle, T., Kleffmann, T., Richards, A. M., & Pemberton, C. J. (2012). Ghrelin signal peptide (GHRSP): Identification, immunoassay and circulating profile. Proceedings of the Medical Sciences Congress (MedSci). (pp. 18). Retrieved from http://www.medscinz.co.nz/QRW2012%20MedSci%20Abstracts.pdf

Conference Contribution - Verbal presentation and other Conference outputs

Algie, M., Jeffs, A., Woolley, A., Kleffmann, T., & Braithwaite, A. (2012, August). Identification of binding partners of the oncoprotein Y-box binding protein (YBX1) in breast cancer cell-line. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

2011

Journal - Research Article

von Zychlinski, A., Kleffmann, T., Williams, M. J. A., & McCormick, S. P. (2011). Proteomics of Lipoprotein(a) identifies a protein complement associated with response to wounding. Journal of Proteomics, 74, 2881-2891. doi: 10.1016/j.jprot.2011.07.008

Wong, C. K., Young, V. L., Kleffmann, T., & Ward, V. K. (2011). Genomic and proteomic analysis of invertebrate iridovirus type 9. Journal of Virology, 85(15), 7900-7911. doi: 10.1128/jvi.00645-11

Park, S. J., Kleffmann, T., & Hessian, P. A. (2011). The G82S polymorphism promotes glycosylation of the receptor for advanced glycation end products (RAGE) at Asn81: A comparison of wild-type RAGE with the G82S polymorphic variant. Journal of Biological Chemistry, 286(24), 21384-21392. doi: 10.1074/jbc.M111.241281

Dickerhof, N., Kleffmann, T., Jack, R., & McCormick, S. (2011). Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain. FEBS Journal, 278(12), 2034-2043. doi: 10.1111/j.1742-4658.2011.08119.x

Kafka, A. P., Kleffmann, T., Rades, T., & McDowell, A. (2011). The application of MALDI TOF MS in biopharmaceutical research. International Journal of Pharmaceutics, 417(1-2), 70-82. doi: 10.1016/j.ijpharm.2010.12.010

Journal - Research Other

Pemberton, C. J., Richards, A. M., Siriwardena, M., Cameron, V. A., Yandle, T. G., Nicholls, M. G., Kleffmann, T., & Ruygrok, P. (2011). [Response to letter regarding article, B-Type natriuretic peptide signal peptide circulates in human blood: Evaluation as a potential biomarker of cardiac ischemia]. Circulation, 123(6), e234. doi: 10.1161/CIRCULATIONAHA.110.003574

Conference Contribution - Published proceedings: Abstract

von Zychlinski, A., McCormick, S., & Kleffmann, T. (2011). The proteome of human Lp(a) points to a role in wound healing. Proceedings of the 10th Human Proteome World Congress (HUPO). Retrieved from https://b-com.mci-group.com/Abstract/Statistics/AbstractStatisticsViewPage.aspx?AbstractID=71070

Conference Contribution - Poster Presentation (not in published proceedings)

Poulter, R., Tan, H.-X., Butler, M., Kleffmann, T., & Wilbanks, S. (2011, June). Using an intein to create a new biosynthetic route to bioactive cyclic peptides. Poster session presented at the Conference on Genome Engineering, Singapore.

Dickerhof, N., Kleffmann, T., Jack, R., & McCormick, S. (2011, September). Bacitracin inhibits the reductive activity of protein disulfide isomerase by forming a disulfide bond with free cysteines in the substrate binding domain. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Conference Contribution - Verbal presentation and other Conference outputs

von Zychilinski, A., Kleffmann, T., & McCormick, S. (2011, September). Proteomics of lipoprotein(a). Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

2010

Journal - Research Article

Siriwardena, M., Kleffmann, T., Ruygrok, P., Cameron, V. A., Yandle, T. G., Nicholls, M. G., Richards, A. M., & Pemberton, C. J. (2010). B-type natriuretic peptide signal peptide circulates in human blood. Evaluation as a potential biomarker of cardiac ischemia. Circulation, 122, 255-264. doi: 10.1161/CIRCULATIONAHA.109.909937

Kafka, A. P., Kleffmann, T., Rades, T., & McDowell, A. (2010). Characterization of peptide polymer interactions in poly(alkylcyanoacrylate) nanoparticles: A mass spectrometric approach. Current Drug Delivery, 7(3), 208-215.

Conference Contribution - Published proceedings: Abstract

Rodger, E., Jones, G., Kleffmann, T., Carne, A., & McCormick, S. (2010). Proteomic analysis of aortae from human lipoprotein(a) transgenic mice indicates an altered metabolic and redox response that precedes atherosclerosis. Proceedings of the Human Proteome World Congress (HUPO): Launch of the Human Proteome Project. (pp. 143). Retrieved from http://www.hupo.org/congress/congress_2010/downloads/HUPO2010%20World%20Congress%20Abstract%20Book.pdf

Kleffmann, T., Siriwardena, M., Ruygrok, P., Cameron, V. A., Yandle, T. G., Nicholls, M. G., Richards, A. M., & Pemberton, C. J. (2010). Characterisation of unusual modifications of the circulating signal peptide of B-type naturetic peptide: A potential biomarker of cardiac ischemia. Proceedings of the 9th Human Proteome World Congress (HUPO): Launch of the Human Proteome Project. (pp. 106). Retrieved from http://www.hupo.org/congress/congress_2010/downloads/HUPO2010%20World%20Congress%20Abstract%20Book.pdf

Guy, P. L., Carne, D. L., & Kleffmann, T. (2010). Rapid identification of a tomato leaf curling virus using mass spectrometry. Proceedings of the 9th Australasian Plant Virology Workshop. (pp. 52). Retrieved from http://www.australasianplantpathologysociety.org.au

2009

Journal - Research Article

Kafka, A. P., Kleffmann, T., Rades, T., & McDowell, A. (2009). Histidine residues in the peptide D-Lys⁶-GnRH: Potential for copolymerization in polymeric nanoparticles. Molecular Pharmaceutics, 6(5), 1483-1491. doi: 10.1021/mp900043e

Kleffmann, T., Jongkees, S. A. K., Fairweather, G., Wilbanks, S. M., & Jameson, G. N. L. (2009). Mass-spectrometric characterization of two posttranslational modifications of cysteine dioxygenase. Journal of Biological Inorganic Chemistry, 14, 913-921. doi: 10.1007/s00775-009-0504-x

Conference Contribution - Published proceedings: Full paper

Kafka, A. P., Kleffmann, T., Rades, T., & McDowell, A. (2009). Co-polymerization of proteins and peptides in poly(alkylcyanoacrylate) nanoparticles. Proceedings of the 36th Annual Meeting & Exposition of the Controlled Release Society. [CD-ROM] St. Paul, MN: CRS. [Full Paper]

Conference Contribution - Published proceedings: Abstract

van Rij, A. M., Philips, L. V., Bateman, E., Madut, D., Kleffmann, T., & Jones, G. (2009). Proteomic comparison of varicose veins in humans and in a new porcine model. Phlebology, 24, (pp. 233-234). doi: 10.1258/phleb.2009.09a003

Rodger, E. J., Jones, G. T., Kleffmann, T., Carne, A., & McCormick, S. P. A. (2009). Proteomic characterisation of aortae from human lipoprotein(a) transgenic mice indicates altered metabolic flux and redox state. Proceedings of the ComBio2009 Conference. [CD-ROM] [Abstract]

Dickerhof, N., von Zychlinski-Kleffmann, A., Kleffmann, T., Jack, R., & McCormick, S. (2009). Using an old antibiotic to lower lipoprotein(a). Proceedings of the ComBio2009 Conference. [CD-ROM] [Abstract]

Conference Contribution - Poster Presentation (not in published proceedings)

Kafka, A., Kleffmann, T., Rades, T., & McDowell, A. (2009, July). Co-polymerization of proteins and peptides in poly(alkylcyanoacrylate) nanoparticles. Poster session presented at the 36th Annual Meeting & Exposition of the Controlled Release Society, Copenhagen, Denmark.

Conference Contribution - Verbal presentation and other Conference outputs

Garama, D., Barker, M., Kleffmann, T., Bremer, P., & Carne, A. (2009, February). Analysis of sea urchin (Evechinus chloroticus) proteome by 2-D page and mass spectrometry. Verbal presentation at the 34th Lorne Conference on Protein Structure and Function, Lorne, Australia.

Kafka, A., Kleffmann, T., Rades, T., & McDowell, A. (2009, February). Co-polymerization in poly(alkylcyanoacrylates): A matter of histidine. Verbal presentation at the New Zealand Chapter of the Controlled Release Society 11th Conference on Formulation and Delivery of Bioactives, Dunedin, New Zealand.

2008

Conference Contribution - Published proceedings: Full paper

Kafka, A. P., Kleffmann, T., Rades, T., & McDowell, A. (2008). The use of MALDI TOF mass spectrometry to identify co-polymerization in PACA nanoparticles. Proceedings of the 35th Annual Meeting & Exposition of the Controlled Release Society. [CD-ROM] St Paul, MN: CRS. [Full Paper]

Conference Contribution - Poster Presentation (not in published proceedings)

Rodger, E. J., Revell, A. F., Jones, G. T., Kleffmann, T., & McCormick, S. P. A. (2008, November-December). Proteomic analysis of livers from mice with an atherogenic lipoprotein profile. Poster session presented at the New Zealand Institute of Chemistry, New Zealand Society for Biochemistry and Molecular Biology and the New Zealand Society of Plant Biologists Combined Conference, Dunedin, New Zealand.

2007

Journal - Research Article

Kleffmann, T., von Zychlinski, A., Russenberger, D., Hirsch-Hoffmann, M., Gehrig, P., Gruissem, W., & Baginsky, S. (2007). Proteome dynamics during plastid differentiation in rice. Plant Physiology, 143, 912-923. doi: 10.1104/pp.106.090738

Clark, M. B., Jänicke, M., Gottesbühren, U., Kleffmann, T., Legge, M., Poole, E. S., & Tate, W. P. (2007). A mammalian gene PEG10 expresses two reading frames by high efficiency -1 frameshifting in embryonic-associated tissues. Journal of Biological Chemistry, 282(52), 37359-37369.

Wirawan, R. E., Swanson, K. M., Kleffmann, T., Jack, R. W., & Tagg, J. R. (2007). Uberolysin: A novel cyclic bacteriocin produced by Streptococcus uberis. Microbiology, 153, 1619-1630.

Conference Contribution - Published proceedings: Abstract

Rodger, E. J., Revell, A. F., Jones, G. T., Kleffmann, T., Kettle, A. J., & McCormick, S. P. A. (2007). Proteomic analysis of livers from mice with an atherogenic lipoprotein profile. Journal of Clinical Lipidology, 1(5), (pp. 417). doi: 10.1016/j.jacl.2007.08.016

2006

Journal - Research Article

Kleffmann, T., Hirsch-Hoffmann, M., Gruissem, W., & Baginsky, S. (2006). plprot: A comprehensive proteome database for different plastid types. Plant & Cell Physiology, 47(3), 432-436.

Conference Contribution - Poster Presentation (not in published proceedings)

Rodger, E. J., Revell, A. F., Jones, G. T., Kleffmann, T., & McCormick, S. P. A. (2006, February). Proteomic analysis of structural arterial proteins in a mouse model of atherosclerosis. Poster session presented at the 31st Lorne Proteins Conference, Lorne, Australia.

2005

Journal - Research Article

Baginsky, S., Kleffmann, T., von Zychlinski, A., & Gruissem, W. (2005). Analysis of shotgun proteomics and RNA profiling data from Arabidopsis thaliana chloroplasts. Journal of Proteome Research, 4(2), 637-640.

von Zychilinski, A., Kleffmann, T., Krishnamurthy, N., Sjolander, K., Baginsky, S., & Gruissem, W. (2005). Proteome analysis of the rice etioplast: Metabolic and regulatory networks and novel protein functions. Molecular & Cellular Proteomics, 4(8), 1072-1084.

2004

Journal - Research Article

Kleffmann, T., Russenberger, D., von Zychilinski, A., Sjolander, K., Gruissem, W., & Baginsky, S. (2004). The arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions. Current Biology, 14, 354-362.

2001

Journal - Research Article

Kollien, A. H., Grospietsch, T., Kleffmann, T., Zerbst-Boroffka, I., & Schaub, G. A. (2001). Ionic composition of the rectal contents and excreta of the reduviid bug Triatoma infestans. Journal of Insect Physiology, 47, 739-747.

Cabral, M. M. O., Azambuja, P., Gottlieb, O. R., Kleffmann, T., Garcia, E. S., & Schaub, G. A. (2001). Burchellin: Effects on Triatoma infestans and on Trypanosoma cruzi within this vector. Parasitology Research, 87, 730-735.

2000

Journal - Research Article

Cabral, M. M., Kollien, A. H., Kleffmann, T., Azambuja, P., Gottlieb, O. R., Garcia, E. S., & Schaub, G. A. (2000). Rhodnius prolixus: Effects of the neolignan burchellin on in vivo and in vitro diuresis. Parasitology Research, 86(9), 710-716.