Equipment access and operating procedures
To use the flow cytometry equipment, you must first undergo training with a Flow Cytometry staff member and be registered. You will then be added to the Flow Cytometry OMNI team on Microsoft Teams, where you can find up-to-date standard operating procedures.
Flow Cytometry OMNI on Microsoft Teams
To request equipment training and access, contact:
Email omni-flow@otago.ac.nz
Cytek Aurora CS
The Cytek Aurora CS is spectral cell sorter cytometer. It detects the whole spectrum of emitted light.
This machine has 5 lasers, including ultraviolet, violet, blue, yellow-green and red. This modern machine is a game changer, enabling large panels to be sorted. With good controls and planning, 30 to 40 colour panels are possible.
Different cell sizes can be sorted, with different sized nozzles: 70, 85, 100 or even 130um.
This cell sorter can sort cells into 1.5mL Eppendorf, 5mL FACS or 15mL Falcon tubes, alongside 96- and 384-well plates. Please ask the OMNI flow team for more information.
There is no fluorochrome guide, as if the fluorochrome can be excited in this cytometer then it can be detected..
BD LSRFortessa
The Fortessa is a 16 channel analyser cytometer, good for multicolour panels up to 10-12 colours.
| Laser | Mirror | Bandpass | Fluorochrome |
|---|---|---|---|
|
488nm Blue | 505 | 530/30 | FITC, GFP, AF488, CFSE, Zombie Green |
| 685 | 710/50 | PerCP-Cy5.5, PerCP, 7AAD | |
|
561nm Yellow/Green | 586/15 | PS-Dazzle 594, PE, AFS46 | |
| 600 | 610/20 | AFS68, AF594, PE-Cy5, Texas Red | |
| 635 | 670/30 | PE-Cy5.5, PE-Fire 640 | |
| 685 | 710/50 | PE-Cy5.5 | |
| 750 | 780/60 | PE-Cy7 | |
|
633nm Red | 670/14 | APC, AF647, APC-Cy5, DRAQ5 | |
| 690 | 730/45 | AF660, AF680, AF700 | |
| 735 | 780/60 | APC-Cy7, APCH7, APC-efluor 780, APC-fire 750, Live/Dead fixable Near IR, Zombie NIR | |
|
405nm Violet | 450/50 | Pac Blue, BV421, AF405, Zombie Violet, DAPI, CFP | |
| 505 | 525/50 | BV510, Zombie Aqua, Zombie Yellow, Live/Dead Fixable Aqua | |
| 600 | 610/20 | Bv605, eFluor 605, Superbright 600 | |
| 630 | 660/20 | Bv650, Superbright 645 | |
| 690 | 710/50 | Bv711, Bv750 | |
| 750 | 780/60 | Bv750, Bv785 |
Cytek Aurora
The Cytek Aurora is spectral analyser cytometer with immense potential. It detects the whole spectrum of emitted light. The machine here has 5 lasers, including ultraviolet, violet, blue, yellow-green and red. This modern machine is a game changer, enabling large panels that you could only previously see using mass cytometry. With good controls and planning, 30 to 40 colour panels are possible.
Due to the fundamental difference between standard cytometry and spectral cytometry, the flow unit offers comprehensive training in spectral cytometry panel design, machine/software use and big data analysis.
This machine has an automated sample loader attached for high throughput analysis. It can hold 96 well plates or up to 40x 5ml FACs tubes. It is also customisable. Please ask the OMNI flow team for more information.
There is no fluorochrome guide, as if the fluorochrome can be excited in this cytometer then it can be detected.