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Histology

Robert Porteous thumb
Robert Porteous

Histology is the study of microscopic anatomy of cells and tissues. Histology services prepare samples for analysis using a variety of techniques for subsequent examination.

The Otago Histology Services Unit is a specialty facility housed in the Department of Pathology in the University of Otago. The unit provides a well-equipped laboratory for performing histological analysis for diagnostic and research purposes, as well as staff with the necessary expertise to offer advice and guidance on histology and cytological techniques.
Read more about:

Whether you would like advice on your research project, or independent use of our facilities, we are here to help.

Initial advice and consultation

Contact for initial advice and consultation:
Email robert.porteous@otago.ac.nz

For advice on the latest academic advances contact:
Dr Tania Slatter
Academic Leader, Histology
Email tania.slatter@otago.ac.nz

Booking our services

For advice on services and costs
Email histology.services@otago.ac.nz
Tel  +64 3 479 7152 

For current clients please bring the completed form with your samples:
OMNI Histology work request form (PDF)

Please complete the online registration form

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Services and techniques

We are happy to discuss your potential project and provide advice and assistance on:

  • Collection of tissue and specimens
  • Storage and processing
  • Strengths and limitations of different histological experimental approaches

We can carry out the 'wet lab' aspects of your project and perform processing, tissue embedding, sectioning, and routine and specialised staining.

With our specialised equipment and expert team of scientists and technicians, we have the flexibility to tailor our histology techniques to suit any research project.

We offer:

  • Advice on fixative use
  • Decalcification
  • Frozen sectioning
  • Histological supplies
  • Histological training and advice
  • Immunohistochemistry
  • Microtomy and cryostat training
  • Opal multiplex fluorescent immunohistochemistry
  • RNAScope
  • Routine and specialist staining
  • Slide scanning
  • Wax processing and sectioning
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Costs

Process

Description

HSD Non-HSD External
Wax processing and embedding Per cassette – by Histology staff $4.00 $12.00 $15.00
Per cassette – by user $3.50 $8.00
Sectioning standard Per block – by Histology staff $6.00 $15.00 $25.00
Per block – by user $4.00 $5.00
Serial sectioning Per block – by Histology staff $15.00 $35.00 $55.00
Per block – by user $11.00 $15.00
H&E staining (up to 19 slides) – by Histology staff $30.00 $50.00 $70.00
(up to 19 slides) – by user $25.00 $30.00
Special stain (up to 19 slides) – by Histology staff $135.00 $195.00 $275.00
(up to 19 slides) – by user $115.00 $115.00
Manual IHC (up to 19 slides) – by Histology staff $140.00 $380.00 $700.00
(up to 19 slides) – by user $60.00 $60.00
Manual ISH (up to 19 slides) – by Histology staff $55.00 $175.00 $335.00
(up to 19 slides) – by user $15.00 $15.00
Full Bond IHC/ISH (up to 19 slides) Routine kits $110.00 $175.00 $195.00
Partial Bond IHC/ISH (up to 19 slides) User supplied kits $90.00 $155.00 $175.00
ddPCR Generation Oil $50.00 $50.00 $50.00
Per 8 well strip (1 to 5 strips) $40.00 $40.00 $40.00
Per 8 well strip (6 to 12 strips) $30.00 $30.00 $30.00
Aperio scanner Staff scanning $20 per hour $80 per hour $120.per hour
User scanning $10 per hour $40 per hour
Versa scanner Staff or user $30 per hour $120 per hour $200 per hour
Grinder/Polisher Users supply consumables and no equipment charge for now

Downloadable table of Histology Charges (PDF)

Notes:

  • Serial sectioning = more than 2 slides of section ribbons
  • Manual IHC – users supply own antibodies
  • Manual ISH – users supply ISH probes
  • Full Bond IHC – Histology will have routine kits available, e.g. DAB
  • Partial Bond IHC – users supply own kits (Histology staff will facilitate sharing costs or on-selling kits where possible)
  • ddPCR – Users purchase bottle of generation oil which can be used for up to 12 strips

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Equipment

If you have previous experience and would prefer independent use of our facilities, we can offer training:
Email histology.services@otago.ac.nz

OMNI equipment use policy (PDF)

Aperio Imaging System

The Aperio Digital Slide Scanning System provides whole slide imaging with 20x and 40x magnification capabilities.

Automatic Immunostainer

The Bond RxM is an open-system immunostainer designed specifically for research. Capabilities include chromogenic and fluorescent immunohistochemistry, in-situ hybridisation and RNA view. All reagents can be provided, with the option of providing your own antibodies. Optimisation of antibodies is available. Slides specifically designed for immunohistochemistry are also provided.

Automatic Staining

If you have large quantities of slides for staining, we have an automatic staining machine capable of staining up to sixty slides at a time, as well as being able to accommodate specific staining protocols if required.

Cryostat

Our cryostat is a Thermo Fisher Microm HM525 Research Crysostat. With this piece of equipment, sections can be cut from any frozen tissue. A disposable blade holder is used, blades, slides and OCT are available if required.

Embedding

Once your tissue is processed to wax, it can be embedded in any orientation you require using our Arcadia Embedding Machine.

Microtomes

The Unit has five rotary microtomes. Two are kept available specifically for our clients' use. All consumables and associated equipment are provided.

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Register for training

If you have previous experience and would prefer independent use of our facilities, we can offer training on different techniques and the use of equipment such as:

  • Immunohistochemistry (double-staining; fluorescent or chromogenic)
  • In-situ hybridisation (fluorescent or chromogenic) resin embedding and cutting
  • Microtomy and cryostat training
  • Routine and specialised staining
  • Slide scanning

Email histology.services@otago.ac.nz

User Group

The User Group consists of members that represent University of Otago departments that use the Histology unit and it advises the OMNI Academic Advisory Group on direction and strategy.

Visit About us for information about how our user groups work and how to make contact.

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